金叶紫穗槐抗寒生理研究

Physiological Research on Cold Resistance of Amorpha fruticosa cv. Jinye

以二年生金叶紫穗槐(Amorpha fruticosa L.cv.Jinye)和紫穗槐(对照)枝条为对象,分别测定自然降温下叶片的电导率和丙二醛、可溶性糖、可溶性蛋白、脯氨酸的含量以及人工低温下的SOD、POD、CAT活性的变化,探讨金叶紫穗槐在低温条件下的适应能力。结果表明,随着自然温度的降低,金叶紫穗槐的丙二醛含量低于对照;渗透调节物质可溶性糖、可溶性蛋白和脯氨酸含量升高,比对照有更强的低温耐受能力;SOD、POD、CAT活性持续升高,高于对照,抵御低温的能力优于对照。不过金叶紫穗槐的相对电导率与对照的差异不显著。

Taking 2-year-old Amorpha fruticosa L. cv. Jinye and A. fruticosa（control） as the research object,relative conductivity,MDA,soluble sugar,soluble protein and proline were measured separately during natural drop in temperature. SOD,POD,CAT were measured separately under artificial low temperature to explore A. fruticosa cv. Jinye adaptability under low temperature. The results showed that with the decreasing of natural temperature,MDA content of A. fruticosa cv. Jinye was lower than that of control; the content of soluble sugar,soluble protein and proline increased,showing strong low temperature tolerance ability than that of control. The activity of SOD,POD and CAT continuously elevated,which were obviously higher than those in the control, the low temperature resisting ability was superior to the control. The relative conductivity of A. fruticosa cv. Jinye was not significantly different to that of control.