雨蛙素及脂多糖诱导小鼠急性胰腺炎效果分析

Effects of acute pancreatitis mouse model induced by caerulein and lipopolysaccharides

目的观察雨蛙素(CAE)诱导小鼠急性胰腺炎模型中胰腺及肺组织损伤时间变化规律;对比雨蛙素及脂多糖(LPS)不同给药方案诱导的小鼠急性胰腺炎模型效果。方法雨蛙素不同给药频次以及雨蛙素联合脂多糖诱导小鼠急性胰腺炎模型,收集小鼠血浆以淀粉-碘比色法检测淀粉酶活性;取胰腺及肺组织行HE染色观察组织损伤严重程度。结果 CAE7组小鼠胰腺及肺组织损伤在4 h达高峰,24 h开始修复,5 d基本恢复正常;造模后4 h,CAE7组和CAE9组小鼠血淀粉酶活性较对照组升高[(6 461±1 078)U/dl比(3 093±331)U/dl;(6 821±495)U/dl比(3 093±331)U/dl,P<0.001],且CAE7+LPS组较CAE9组更高[(8 912±465)U/dl比(6 821±495)U/dl,P<0.001],CAE7组和CAE9组小鼠胰腺组织病理评分较对照组升高(4.750±0.524比0±0;4.917±0.664比0±0,P<0.001),且CAE7+LPS组较CAE9组更高(7.167±0.258比4.917±0.664,P<0.001)。结论雨蛙素50μg/kg连续7次给药可诱导出小鼠急性胰腺炎模型,增加给药频次未见胰腺组织损伤加重,而联用脂多糖可加重急性胰腺炎模型严重程度。

Objective To observe the time dependent changes of pancreas and lung injury in mice acute pancreatitis induced by caerulein,and compare the effects of mice acute pancreatitis model induced by different injection protocols of caerulein and lipopolysaccharides. Methods Acute pancreatitis were induced by different injection frequency of caerulein or caerulein combined with lipopolysaccharides,and plasma amylase activity was detected by starch-iodine colorimetry,and pancreas and lung injury severity was observed on paraffin section after HE staining. Results The injury of pancreas and lung in mice acute pancreatitis induced by caerulein was most severe at4 h,which began to repair at 24 h,and basically returned to normal at 5 d. 4 h after last injection,the amylase activity in CAE7 group and CAE9 group was higher than that in the control group（ 6 461 ± 1 078 U/dl vs 3 093 ±331 U/dl; 6 821 ± 495 U/dl vs 3 093 ± 331 U/dl,all P〈0. 001）,and CAE7 ＋ LPS group was higher than in CAE9 group[（ 8 912±465） U/dl vs（ 6 821±495） U/dl,P〈0. 001],the histological score in CAE7 group andCAE9 group was higher than that in the control group（ 4. 750 ± 0. 524 vs 0 ± 0; 4. 917 ± 0. 664 vs 0 ± 0,all P 0. 001）,and that in CAE7 ＋ LPS group was higher than in CAE9 group（ 7. 167 ± 0. 258 vs 4. 917 ± 0. 664,P 0. 001）. Conclusions Mice acute pancreatitis can be successfully induced by 7 i. p. injections of caerulein（ 50 μg/kg body weight） at 1-hour intervals,caerulein combined with lipopolysaccharides.