柿叶黄酮在实验性口腔溃疡中的抗氧化抗炎作用研究

Study on the antioxidant and anti-inflammatory effects of persimmon leaf flavonoids in experimental oral ulcer

目的探讨柿叶黄酮在口腔溃疡大鼠中的抗氧化抗炎作用。方法建立口腔溃疡大鼠模型(醋酸法),用桂林西瓜霜和不同剂量(20 mg·kg^(-1),40 mg·kg^(-1),80 mg·kg^(-1))的柿叶黄酮进行干预。观察血清和溃疡组织中的超氧化物歧化酶(SOD)和丙二醛(MDA)的变化;H-E染色观察组织病理形态学变化。结果柿叶黄酮(20 mg·kg^(-1),40 mg·kg^(-1),80 mg·kg^(-1))可提高模型大鼠血清和溃疡组织中SOD活性、降低MDA含量(P<0.05);桂林西瓜霜和减轻溃疡组织的炎性浸润。结论柿叶黄酮对醋酸诱导的口腔溃疡有一定的治疗作用,其机制与抗氧化损伤和降低炎症反应有关。

Objective： To investigate the antioxidant and anti-inflammatory effects of persimmon leaf flavonoids on oral ulcer in rats. Methods： The oral ulcer model was induced by acetic acid,and was used for intervention by Guilin watermelon frost and different doses of PLF（ 20 mg·kg^（-1）,40 mg·kg^（-1）,80 mg·kg^（-1））. The changes of superoxide dismutase（ SOD） and malondialdehyde（ MDA） in serum and ulcer tissues were observed. The changes of histopathology were observed by H-E staining. Results： PLF（ 20 mg·kg^（-1）,40mg·kg^（-1）,80 mg·kg^（-1）） increased the activity of SOD and decreased the content of MDA in serum and ulcer tissue（ P〈0. 05）; Guilin watermelon frost and PLF could improve the inflammatory infiltration of ulcer tissue. Conclusion： PLF has certain therapeutic effects on oral ulcer induced by acetic acid,and the mechanism may be related to improving oxidative damage and reducing inflammatory reaction.