粉末冶金制备TNZS基生物材料的体外组织相容性

Histocompatibility in Vitro of TNZS-Based Biomaterials Prepared by Powder Metallurgy

采用高能球磨与冷压烧结相结合的粉末冶金法制备了TNZS、5%TiO_2/TNZS及5%HA/TNZS(质量分数,下同)生物材料,并研究了TiO_2和HA的添加对TNZS体外组织相容性的影响。结束表明:3种TNZS基生物材料均无细胞毒性;5%TiO_2/TNZS和5%HA/TNZS表面在1,3,5和7 d的细胞相对增殖抑制率CPIR值大幅低于TNZS组,TiO_2和HA的添加显著提高了TNZS材料表面的细胞增殖速度,增强了其细胞增殖能力,更有助于诱导成骨细胞的体外增殖;3种TNZS基材料表面贴附的成骨细胞伪足伸展状态良好,而5%TiO_2/TNZS表面贴附的成骨细胞分布更加均匀。

TNZS,5%TiO_2/TNZS and 5%HA/TNZS（mass fraction） bio-medical materials were prepared by powder metallurgy combining high-energy milling with cold pressed sintering. Histocompatibility in vitro of the three TNZS-based materials was studied. The results show that the three materials have no cytotoxicity; CPIR on the surface of 5%TiO_2/TNZS and 5%HA/TNZS for 1,3,5 and 7 d is significantly lower than that of TNZS group,indicating that the addition of TiO_2 and HA can accelerate the cell proliferation on the surface of TNZS material and favors induction of proliferation of osteoblasts in vitro; pseudopodium extension of osteoblasts attached to the surface of the three materials are in good condition; the distribution of osteoblasts attached to the surface of 5%TiO_2/TNZS is more uniform.