玉米耐盐基因ZmHKT1;5在烟草中的功能验证

Overexpression of ZmHKT1;5 Enhances Tolerance to Salt Stress in Nicotiana tabacum

HKT类基因是与植物耐盐性密切相关的一类基因。在作物中HKT蛋白可通过排出Na+来维持植物体内的Na^+/K^+平衡,从而影响植物耐盐性。通过在烟草中过表达玉米ZmHKT1;5基因,验证该基因具有提高植物耐盐性的作用。结果表明,过表达ZmHKT1;5基因的T0代材料即显示出叶片耐盐能力的明显提高;T2代转基因株系种子在含盐培养基上的发芽能力明显强于野生型材料,T2代转基因株系幼苗阶段的耐盐能力也得到了明显的提高。通过比较在盐胁迫后2月龄的转基因材料和野生型材料的生理指标,发现野生型材料中MDA和H_2O_2的含量相较转基因材料发生了更为明显的上升,说明转基因材料中过表达ZmHKT1;5基因有效降低了盐胁迫引起的过氧化物积累。综合转基因验证的结果,证明ZmHKT1;5基因具有提高植物耐盐性的作用。

The HKT like genes are closel tolerance by discharging Na ＋ to maintain Na y related to salt tolerance in plants. HKT proteins can affect the salt Na＋/K＋ balance. In this study, the maize ZmHKT1;5 gene was overexpressed in tobacco（Nicotiana tabacum） to verify that the gene also had the effect of improving plant salt tolerance. The results showed that the TO leaves of overexpressing ZmHKT1;5 showed a significant increase in salt tolerance. The germination ability of T2 seeds was much better than that of wild-type materials under salt stress. The results also showed that the content of MDA and H2O2 in the wild-type materials were higher than those in the transgenic, which indicated that overexpressing ZmHKT1,5 gene effectively reduced the peroxide accumulation caused by salt stress in the transgenic materials. Based on the results of our research, it was proved that the ZmHKT1;5 gene could improve the salt tolerance in plants.