西藏加查县濒危药材桃儿七真叶分化研究

Study on Leaf Differentiation of Endangered Medicinal Materials-Sinopodophyllum hexandrum

目的:探索一种促进野生桃儿七真叶分化的一种方法。方法:以采自西藏加查县经过自然休眠的野生桃儿七种子为试材,首先分别用0、25、50、100、150、200 mg·L^(-1)的GA进行处理,筛选出最适合真叶分化浓度;用0、25、50、100、150、200 mg·L^(-1)的6-BA进行处理,筛选出最适合真叶分化的浓度;再用100 mg·L^(-1)GA与不同质量浓度6-BA配比对桃儿七真叶分化的影响。结果:100 mg·L^(-1)质量浓度GA处理下真叶的分化率最高,分别为84.1%;同时真叶畸形率仅为3.3%,是比较理想的处理浓度。结论:在桃儿七种子萌发过程中添加适量的GA、6-BA,及二者的配比,能够有效的提高桃儿七真叶的分化率,但过高的激素含量会造成较高的真叶畸形率,畸形苗不能够存活。

Objective： The study is aimed to find a method for leaf differentiation of SinopodophyUum hexandrum. Methods： The natural dormant seeds of wild S. hexandrum harvested in Jiacha of Tibet were collected. GA was added to screen the optimal concentration, then 6-BA was used to screen the optimal concentration for study of leaf differentia- tion of S. hexandrum. Results： The leaf differentiation of S. hexandrum was 84. 1% under 100 mg ·L^-1 of GA, and the malfor- mation rate was 3.3%. Conclusion： GA and 6-BA could promote leaf differentiation of S. hexandrum, however high concentration of plant hormones may result in the malformation of the leaf.