摘要
With the increasingly promising role of nanomaterials in tissue engineering and regenerative medicine, the interaction between stem cells and nanoparticles has become a critical focus. The entry of nanoparticles into cells has become a primary issue for effectively regulating the subsequent safety and performance of nanomaterials in vivo. Although the influence of nanomaterials on endocytosis has been extensively studied, reports on the influence of stem cells are rare.Moreover, the effect of nanomaterials on stem cells is also dependent upon the action mode. Unfortunately, the interaction between stem cells and assembled nanoparticles is often neglected. In this paper, we explore for the first time the uptake of γ-Fe2O3 nanoparticles by adipose-derived stem cells with different passage numbers. The results demonstrate that cellular viability decreases and cell senescence level increases with the extension of the passage number. We found the surface appearance of cellular membranes to become increasingly rough and uneven with increasing passage numbers. The iron content in the dissociative nanoparticles was also significantly reduced with increases in the passage number. However, we observed multiple-passaged stem cells cultured on assembled nanoparticles to have similarly low iron content levels. The mechanism may lie in the magnetic effect of γ-Fe2O3 nanoparticles resulting from the field-directed assembly. The results of this work will facilitate the understanding and translation of nanomaterials in the clinical application of stem cells.
随着纳米材料在组织工程和再生医学中越来越多的应用,干细胞和纳米材料之间的相互作用成为关键环节,而纳米颗粒进入细胞是关系到纳米材料安全性和干细胞命运调控的首要问题.干细胞传代是其应用中必不可少的过程,但关于传代代数对干细胞摄取纳米颗粒的影响的研究还较少.此外,干细胞和纳米材料的相互作用还与纳米颗粒的存在方式有关.本文在玻璃片上组装了条带状的γ-Fe_2O_3纳米颗粒组装结构,并在该表面上培养SD大鼠脂肪间充质干细胞,然后研究了不同代数的干细胞对组装和游离的纳米颗粒的吞噬情况.结果发现,随着细胞代数增加,细胞活力降低,细胞衰老水平增加,并且细胞膜的表面呈现出粗糙和不均匀的形貌.当与游离的γ-Fe_2O_3纳米颗粒共培养时,细胞内铁含量随着代数的增加而减少,但在组装体上培养的不同代数的细胞具有相似的铁含量,并且胞内铁含量极少.另外,磁感应蛋白的表达表明磁性纳米颗粒的组装体对细胞有磁效应.该研究表明,细胞代数的选择对研究颗粒内化实验是至关重要的,细胞代数应该作为细胞摄取实验的一个重要考虑因素.
基金
supported by the National Basic Research Program of China(2013CB733801)
the National Key Research and Development Program of China(2017YFA0104301)
thankful to the supports from the Fundamental Research Funds for the Central Universities
