睡安片对咖啡因诱导斑马鱼失眠症的改善作用

Improvement of Shui'an Tablets on insomnia of zebra fishes caused by caffeine

目的观察睡安片对咖啡因诱导失眠症斑马鱼的镇静作用和睡眠改善作用,评价睡安片对失眠症的疗效。方法采用200μmol/L咖啡因诱导活体斑马鱼建立失眠症模型,并确定睡安片的最大非致死浓度(MNLC)。睡安片选取MNLC(1 200μg/m L)、1/3 MNLC(400μg/m L)、1/10 MNLC(120μg/m L)3个质量浓度,同时设置对照组、模型组和氯化锂(500μg/m L)组,每组均处理30尾斑马鱼幼鱼,使用行为分析仪测量斑马鱼的运动距离和处于失眠状态的时间,计算镇静作用和睡眠改善作用。结果睡安片的MNLC为1 200μg/m L。120、400、1 200μg/m L睡安片组与模型组比较,运动距离均显著减少,差异具有极显著性(P<0.001),镇静作用分别增加0.69、1.66、2.74倍。除120μg/m L质量浓度外,400、1 200μg/m L组与模型组比较,失眠时间显著减少,差异具有极显著性(P<0.001),睡眠改善作用分别增加1.63、2.65倍。结论睡安片对咖啡因诱导失眠症斑马鱼具有明显的镇静和睡眠改善作用,呈现明显的浓度相关性。

Objective To observe the sedative effect and sleep improvement of Shui＆#39;an Tablets on zebra fishes with insomnia caused by caffeine, and to evaluate the effect of Shui＆#39;an Tablets on insomnia.Methods The insomnia model was established by 200μmol/L caffeine in living zebra fishes, and the maximum non-lethal concentration （MNLC） of Shui＆#39;an Tablets was determined. All zebra fishes were randomly divided into control group, model group, lithium chloride （500μg/mL） group, and Shui＆#39;an Tablets 1/10 MNLC （120μg/mL）, 1/3 MNLC （400μg/mL）, and MNLC （1200μg/mL） groups, and each group had 30 zebra fishes. The distance of movement and the time of insomnia were measured with behavioral analyzer, and sedative effect and sleep improvement of Shui＆#39;an Tablets on zebra fishes was calculated.Results The maximum non-lethal concentration （MNLC） of Shui＆#39;an Tablets was 1200μg/mL. Compared with the model group, the exercise distances of Shui＆#39;an Tablets 120, 400, and 1200μg/mL groups were significantly decreased （P 〈 0.001） and sedative effects were 0.69, 1.66, and 2.74 times, respectively. Compared with the model group, the times of insomnia of Shui＆#39;an Tablets 400 and 1200μg/mL groups were significantly decreased （P 〈 0.001）, and the improvements of sleep were0.02, 1.63, and 2.56 times, respectively.Conclusion Shui＆#39;an Tablets have obvious sedative and sleep improvement effects on zebra fishes with insomnia caused by caffeine, and show significant concentration-dependence.