花生种子萌发基因AhSGR的表达分析

Expression Analysis of Seed Germination Related Gene AhSGRin Peanut(Arachis hypogaea L.)

种子萌发是一个复杂的多步骤过程,而与花生种子萌发相关基因的研究鲜见报道。本研究以花育52号为试材,根据花生吸胀休眠和休眠释放转录组测序结果获得了一个与花生种子萌发相关的基因AhSGR。AhSGR基因cDNA全长1338bp,开放阅读框(ORF)为885bp,编码295个氨基酸,该基因编码未知蛋白,在44~122AA处含有DOG1保守结构域,分子量33.39kD,等电点为5.04,定位于细胞核内,未发现信号肽及其剪切位点,推测AhSGR可能是转录因子。荧光定量检测表明该基因与花生种子萌发密切相关。

Seed germination is a complex and multistep process. There are httLe reports on genes related to peanut seed germination. To research important functional genes on control of seed germi- nation, the full-length cDNA sequence of a gene related to seed germination （AhSGR） was screened from seed of peanut cultivar Huayu52 by RNA-seq. The whole sequence of AhSGR was 1338bp and its open reading frame was 885bp, encoding a 295 amino acid residuces. AhSGR encoded unknown protein which had DOG1 conserved domain in 44-122AA, a molecular weight of 33.39 kD and pI of 5. 04, which was located in nucleus. It had no signal peptide and splice site. According to allignment sim- ilarity by Blastp, We speculated that AhSGR may be a transcription factor. The results of real-time quanti- tative PCR showed that the expression of AhSGR gene was closely correlated to the seed germination.