摘要
以玉米蛋白粉为原料,采用微波预处理协同碱性蛋白酶水解制备抗氧化肽,并研究其对血管紧张素转化酶(ACE)的抑制效果。通过单因素试验,确定出最佳的微波预处理条件为:微波功率400 W、微波时间2.0 min,通过中心组合设计及响应面分析确定了最佳水解条件为:碱性蛋白酶添加量9 000 U/g、酶解时间2.5 h、酶解温度45℃、酶解p H 8.9,在此条件下,玉米蛋白粉平均水解度为14.13%,同时得到的玉米抗氧化肽对ACE有一定的抑制作用,其半抑制浓度(IC50)为4.99 mg/m L。
With corn gluten meal as raw material, microwave pretreatment assisted alkaline protease was adopted to prepare antioxidant peptides, and the inhibitory effects of antioxidant peptides against angiotensin converting enzyme were studied. The optimum microwave pretreatment conditions were determined by single factor experiments：microwave power 400 W, microwave time 2.0 min. The optimized hydrolytic conditions by alkaline protease were obtained by center combination design and response surface analysis as follows：alkaline protease addition 9 000 U/g,enzymolysis time 2.5 h, enzymolysis temperature 45 ℃ and p H 8.9. Under these conditions, the mean degree of hydrolysis of corn gluten meal reached 14.13%, and the corn antioxidant peptides obtained showed certain inhibitory activity to angiotensin conversion enzyme and its IC50 was 4.99 mg/m L.
作者
赵凤影
关海宁
于新月
张波
张菊灵
张子艳
ZHAO Feng-ying GUAN Hai-ning＊ YU Xin-yue ZHANG Bo ZHANG Ju-ling ZHANG Zi-yan(College of Food and Pharmaceutical Engineering, Suihua College, Suihua 152061, China)
出处
《保鲜与加工》
CAS
北大核心
2017年第5期40-45,共6页
Storage and Process
基金
黑龙江省大学生创新创业训练计划项目(201510236002)
关键词
玉米蛋白粉
抗氧化肽
微波
酶解
血管紧张素转化酶
抑制
corn gluten meal
antioxidant peptides
microwave
enzymolysis
angiotensin conversion enzyme
inhibit
