胰岛素受体在猕猴腰段脊髓及相应背根神经节中的分布

Distribution of insulin receptor in L4-L6 segments of spinal cord and corresponding dorsal root ganglion in the rhesus monkey

目的:观察胰岛素受体在猕猴脊髓腰段第4~6(L4~L6)节段及相应节段背根神经节(dorsal root ganglion,DRG)中的形态学分布特点。方法:取1只成年猕猴的L4~L6节段脊髓并留取相应节段的DRG置于固定液中。脊髓冷冻水平切片(30μm)平均分为4组,分别行Nissl染色、荧光免疫组织化学染色、免疫组织化学染色和对照组处理,再于光学显微镜下观察;DRG平均分为2组,分别行免疫组织化学染色和对照组处理,再于光学显微镜下观察。结果:胰岛素受体分布于猕猴腰段脊髓L4~L6节段及相应节段的DRG中:(1)在腰段脊髓L4~L6节段,背角可见胰岛素受体阳性神经元胞体及终末,并大量分布于背角浅层(集中在第I、II层),腹角也可观察到散在胰岛素受体阳性神经元;(2)在L4~L6节段对应的DRG中,胰岛素受体阳性产物主要集中分布于中型(20μm<直径≤40μm)、小型(直径≤20μm)神经元。结论:本研究为进一步研究灵长类动物脊髓和DRG中胰岛素受体的功能提供形态学依据。

Objective ： To observe the distribution of insulin receptor （IR） in lumbar 4-6 （IA-L6） segments of rhesus monkey spinal cord and corresponding dorsal root ganglions （DRGs）. Methods ： IA-L6 segments of spinal cord and cor- responding DRGs of an adult rhesus monkey were collected and put in fixation liquid. The spinal cord was cut into hori- zontal slices in 30 I^m thickness. Sections were divided averagely into 4 groups, for Nissl＇s staining, fluorescence immu- nohistochemistry, immunohistochemistry and control group treatment respectively and all of them were observed with light microscopy. DRG were divided averagely into 2 groups, for immunohistochemistry and control group treatment respectively and both of them were observed with light microscopy. Results： IRs were distributed in the IA-L6 segments of the rhesus monkey spinal cord and corresponding DRG： （ 1 ） in the IA-L6 segments of spinal cord, IR-positive cell bodies and nerve endings could be found in the dorsal horn, and they were distributed massively in superficial layers （especially in lamina I and II） of dorsal horn, while IR-positive neurons are distributed diffusedly in the ventral horn. （2） In the L4-L6 corre- sponding DRGs, IR-positive products were mainly distributed in the medium-sized （20 μm 〈 diameter ≤40μm） and small-sized （diameter≤20 μm） neurons. Conclusion： This research provides morphological evidences for the further studies of the function of the IR distributed in primate spinal cord and DRG.