丙烯酰胺暴露对幼鼠胼胝体PLP和MBP表达的影响

Effect of acrylamide on the expression of PLP and MBP in the corpus callosum of young rats exposed to acrylamide

目的:检测断乳期幼鼠胼胝体髓鞘蛋白PLP和MBP的表达,探讨丙烯酰胺(acrylamide,ACR)染毒对幼鼠胼胝体部髓鞘发育的影响。方法:断乳期幼鼠随机分为对照组(0 mg/kg)、低(18 mg/kg)和高(36 mg/kg)剂量组,每组12只,从出生后第22~42 d进行灌胃染毒。观测幼鼠步态的变化,用免疫组化方法和免疫荧光双标记法检测幼鼠胼胝体髓鞘蛋白脂蛋白(myelin PLP,PLP)和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果:ACR染毒后幼鼠的步态评分均增加,差异有统计学意义(P<0.01),免疫组织化学检测结果显示,与对照组相比较,ACR高剂量组幼鼠大脑PLP和MBP表达减少,差异有统计学意义(P<0.05)。免疫荧光双标技术检测结果与免疫组织化学检测结果一致,即ACR染毒后胼胝体PLP和MBP均表达减少。结论:ACR染毒可能会通过减少PLP和MBP的表达,抑制胼胝体髓鞘的形成而影响神经系统发育。

Objective： To explore the effect of acrylamide （ACR） treatment on myelin development in young rats＇corpus callosum by detecting the expression of myelin PLP and myelin basic protein （MBP）. Methods： Weaning rats were randomly divided into low （18 mg/kg） , high （36 mg/kg）dose group and the control group （0 mg/kg） , 12 rats in each group were exposure to toxicant form 22 days to 42 days after birth. Research content including observed the change of the gait, and the expression of PLP and BMP in young rats＇brain were measured by immunohistochemistry and immunofluores- cence double labeling. Results： The gait scores of ACR infected young rats were significantly increased （P 〈 O. 01 ）. Immunohistochemical detection results showed that compared with control group, the expression of PLP and MBP in young rats＇corpus callosum in ACR high dose group was markedly reduced （ P 〈 0.05 ）. Immunofluorescence double labeling test result was consistent with the testing results of immunohistochemisry that PLP and MBP were reduced after ACR treat- ment. Conclusion： ACR treatment may inhibit the formation of the myelin of young rats＇corpus callosum by reducing the expression of MBP and PLP.