转化生长因子β1及其信号转导分子Smad2在病变肾小球中的表达及其意义

The expressions of TGF-β1 and Smad 2 mRNA on diseased glomeruli and their significance in the development of glomerulosclerosis

目的 探讨转化生长因子 β1(TGF β1)及其信号转导分子Smad 2在肾小球肾炎中的表达及其意义。方法 以 11例肾小球轻微病变为对照组 ,对各种不同组织学类型的IgA肾病 4 0例、膜性肾炎 10例、硬化性肾炎 11例的肾穿刺活检标本 ,分别行原位分子杂交检测肾小球Smad 2mRNA的表达和免疫组织化学ABC方法观察TGF β1、Ⅳ型胶原及纤连蛋白在肾小球中的染色强度 ,并对其检测结果作图像分析处理。结果 除轻微病变型IgA肾病的Smad 2mRNA表达外 ,其他病理类型肾炎中病变肾小球TGF β1蛋白和Smad 2mRNA的表达均高于对照组 ,且两者在肾小球中的表达与Ⅳ型胶原、纤连蛋白在肾小球中的沉积呈正相关。结论 TGF β1及其信号转导分子Smad 2可能参与了病变肾小球细胞外基质的过量积聚 。

Objective To explore the expressions of transforming growth factor β1 (TGF β1) and its signaling transduction molecule Smad 2 and their significance in the development of glomerulosclerosis Methods Using in situ hybridization and immunohistochemistry to detect Smad 2 mRNA expression and TGF β1, collagen Ⅳ, fibronectin expression in renal biopsies from 61 cases with a spectrum of glomerulonephritis including IgA nephropathy (40 cases), membranous glomerulonephritis (10 cases) and sclerosing glomerulonephritis (11 cases), compared with 11 cases of glomerular mild lesion with image analysis system Results With the exception of Smad 2 mRNA expression in mild type IgA nephropathy, all other types of diseased glomeruli showed increased expression of both TGF β1 and Smad 2 mRNA when compared with the 11 cases of mild glomerular lesions The expressions of glomerular TGF β1 and Smad 2 mRNA positively correlated with collagen Ⅳ and fibronectin deposition in the glomeruli Conclusions TGF β1 and Smad 2 may be involved in the excessive deposition of glomerular extracellular matrix and play an important role in the development of glomerulosclerosis