上皮性钙黏附蛋白和β-连环素的表达与非小细胞肺癌预后的关系

A study of E-cadherin and β-catenin expression and their correlation with prognosis of nonsmall cell lung carcinoma

目的 探讨上皮性钙黏附蛋白 (E cad)及 β 连环素 (β cat)在非小细胞肺癌 (NSCLC)中的表达及其与分型、分化、转移和预后的关系。方法 免疫组织化学 (SP法 )和原位杂交法。结果 免疫组织化学 :10 1例NSCLC中E cad及 β cat的阳性率分别为 6 8 3%和 81 2 % ,异常表达率分别为6 1 4 %和 6 4 4 %。E cad和 β cat的异常表达率与组织类型无关 (P >0 0 5 ) ,而与分化程度有相关性 (P<0 0 5 ) ,在有淋巴结转移病例中E cad及 β cat的异常表达率明显高于无转移病例 (P <0 0 5 )。E cad和 β cat异常表达病例的平均生存时间和 5年生存率明显低于正常表达病例。原位杂交 :NSCLC中E cad和 β catmRNA的阳性率分别为 38 9%和 4 7 2 % ,与蛋白正常表达一致率分别为 78 6 %和 82 4 %。结论 检测β cat或E cad/ β cat的共同表达将是指导临床治疗及估计预后的有意义指标 ,可应用于临床预后的综合评估。

Objective To investigate the protein and mRNA expression of E cadherin and β catenin in nonsmall cell lung carcinoma (NSCLC) and to find their correlation with histological type, differentiation, metastasis and prognosis Methods High sensitive S P immunohistochemical method and in situ hibridization were used to detect the protein and mRNA expression of E cadherin and β catenin Results Immunohistochemistry revealed that among the 101 cases, the positive rates of E cadherin and β catenin were 68 3% and 81 2% respectively The abnormal expression rates of these two proteins were 61 4% and 64 4% respectively There was no significant relationship between E cadherin and β catenin staining and histological type of the tumor ( P >0 05). However, there was a statistically significant difference between well and moderately differentiated cells and poorly differentiated cells ( P <0 05) In cases with lymphatic metastasis, the abnormal expression rates of E cadherin and β catenin were significantly higher than those in nonmetastatic cases ( P <0 05) The mean survival time in cases with abnormal E cadherin and β catenin expression were significantly shorter than that in cases with the expression grading (++)～(+++) In situ hybridization showed that in NSCLC,the positive rate of E cadherin and β catenin mRNA was 38 9% and 47 2% respectively Their concordant rates with (++)～ (+++) protein expression were 78 6% and 82 4%, respectively Conclusions The concordant rate of E cadherin and β catenin mRNA and protein expression was relatively high They can be used as markers of prognosis of NSCLC in clinical practice