电子束辐照与邻酪氨酸产率的剂量关系

Relationship Between o-Tyrosine Content and Irradiation Dose in Phenylalanine with the Electron Beam Irradiation

阐明了辐照剂量与邻酪氨酸转化率的关系。以邻酪氨酸的前体苯丙氨酸为研究对象,给予不同电子束辐照剂量,使用液相色谱-串联质谱联用法同时测定3种酪氨酸同分异构体和苯丙氨酸,探讨了在无基质干扰条件下辐照剂量与邻酪氨酸转化率的关系,以及苯丙氨酸在不同辐射剂量下转化为邻酪氨酸的效率。结果表明:在辐照剂量为1~10kGy时,邻酪氨酸的转化率为0.019%~0.105%,远高于样品基质中邻酪氨酸的转化率。

In this study,the relationship between the contents of o-tyrosine in phenylalanine and the electron beam irradiation dose was studied.Phenylalanine,as the precursor of otyrosine,was irradiated by electron beam with different irradiated dose,then a stable LCMS/MS method was developed for the simultaneous determination of three tyrosine isomers and phenylalanine,the relationship between the irradiation dose and o-tyrosine content was investigated without the interference of matrix,and the efficiency of phenylalanine conversion to o-tyrosine was analyzed under different irradiation doses.The results showed that the conversion of phenylalanine to o-tyrosine at different irradiated dose was of 0.019%—0.105% at 1—10 kGy dose in a dose-dependent manner,much more higher than that in seafood which indicated that the matrix of seafood would produce effect on the conversion of phenylalanine to o-tyrosine.