摘要
目的建立HPLC法同时测定党参固本丸(地黄、熟地黄、党参等)中梓醇、麦角甾苷、焦地黄苯乙醇苷B1、角胡麻苷、党参炔苷、甲基麦冬黄烷酮A、甲基麦冬黄烷酮B的含有量。方法该药物50%甲醇提取液的分析采用Agilent TC-C18色谱柱(200 mm×4.6 mm,5μm);以乙腈-0.2%磷酸为流动相,梯度洗脱;检测波长210、330、269、296 nm;体积流量1.0 m L/min;柱温30℃。结果 7种成分在各自范围内线性关系良好(r≥0.999 2),平均加样回收率96.96%~99.65%,RSD 0.88%~1.74%。结论该方法简便准确,重复性好,可用于党参固本丸的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martynoside,lobetyolin,methylophiopogonanone A and methylophiopogonanone B in Dangshen Guben Pills (Rehmanniae Radix,Rehmanniae Radix Preparata,Codonopsis Radix,etc.).METHODS The analysis of 50% methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent TC-C(18) column (200 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2% phosphoric acid flowing at 1.0 m L/min in a gradient elution manner,and the detection wavelengths were set at 210,330,269 and 296 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r≥0.999 2),whose average recoveries were 96.96%-99.65% with the RSDs of 0.88%-1.74%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dangshen Guben Pills.
出处
《中成药》
CAS
CSCD
北大核心
2017年第10期2069-2072,共4页
Chinese Traditional Patent Medicine
关键词
党参固本丸
化学成分
HPLC
Dangshen Guben Pills
chemical constituents
HPLC