4种新型蛋白激酶C同工酶在不同肾小球疾病中的表达

Expression of 4 novel protein kinase C isozymes in difference glomerular diseases

目的探讨4种新型蛋白激酶C同工酶(PKC-δ、-ε、-η、-θ)在肾小球疾病中的作用。方法收集50例肾小球疾病(肾小球轻微病变、膜性肾病、局灶节段性肾小球硬化症、IgA肾病、狼疮性肾炎)患者肾活检标本及6例正常肾组织,免疫组化检测PKC-δ、-ε、-η、-θ表达。结果病变肾组织中PKC-δ、-ε、-η、-θ表达部位与正常肾组织相同。PKC-δ在狼疮性肾炎肾小球中表达高于正常肾组织(P<0.01),而病变肾组织中PKC-ε、-η、-θ表达与正常肾组织差异无统计学意义(P>0.05)。结论 PKC-δ、-ε和-θ可能参与肾小球损伤和肾小管间质纤维化,而PKC-η可能仅参与肾小球损伤。

Objective To investigate the role of 4 novel protein kinase C isozymes （PKC-δ, -ε, -ηand -θ） in glomerular diseases. Methods Expression of PKC-δ, -ε, -η and - θwas detected by immunohistochemistry in 50 biopsied kidney speci- mens of glomerular diseases （minor glomerular abnormalities, membranous nephropathy, focal segmental glomerulosclerosis, IgA nephropathy, and lupus nephritis） and 6 normal kidney specimens. Results In normal renal tissues, PKC-δ, -ε, and -θ were detected in glomeruli and RTECs, while PKC-η was only detected along glornerular capillary loops. None of them were detected in renal interstitium. The distribution of PKC-δ, -ε, -η and -θ expression in glomerular diseases was similar to that of normal kidney. Expression of PKC-δ was higher in lupus nephritis than in normal kidney （P〈0.01）, while expression of PKC-ε, -η and -θ showed no difference between glomerular diseases and normal kidney （P〉0.05）. Conclusion PKC-δ, -ε and -θ may contribute to the glomerular damage and tubulointerstitial fibrosis, while PKC-η may be involved in the glomerular damage.