摘要
目的探讨白介素-6(IL-6)在人结肠癌细胞株奥沙利铂(L-OHP)耐药中的作用。方法通过L-OHP诱导建立人结肠癌L-OHP耐药细胞株Lo Vo/L-OHP,分别用MTT法、免疫荧光及蛋白印迹检测细胞生长、IL-6表达。L-OHP联合IL-6或IL-6R抗体作用后,MTT法和克隆形成实验检测细胞增殖。结果与Lo Vo细胞相比,Lo Vo/L-OHP细胞的耐药指数为10.4,IL-6表达上调。阻断IL-6可增强L-OHP对耐药细胞的杀伤作用,但阻断IL-6R后耐药细胞对L-OHP敏感性无明显改变。结论 IL-6过表达与Lo Vo细胞L-OHP耐药相关,阻断IL-6而非IL-6R可增强耐药细胞对L-OHP敏感性。
Objective To investigate the role of IL-6 in oxaliplatin （L-OHP）-resistant LoVo human colon cancer cells. Methods L-OHP-resistant LoVo/L-OHP cells were established by L-OHP induction, and cell growth and IL-6 expression were detected by MTT assay, immunofluorescence and Western blot, respectively. After treated with L-OHP and IL-6 or IL-6R antibody, cell proliferation was determined by MTT and colony forming assays. Results Compared with LoVo ceils, resis- tance index against L-OHP was 10.4 fold, and IL-6 expression was upregulated in LoVo/L-OHP cells. IL-6 blockade enhanced the killing effect of L-OHP on the resistant cells, but IL-6R blockade had no impact. Conclusion IL-6 overexpression is associated with L-OHP resistance in LoVo cells. IL-6 blockade, but not IL-6R, can increase the sensitivity of resistant ceils to L-OHP.
出处
《广东医科大学学报》
2017年第4期337-341,共5页
Journal of Guangdong Medical University
基金
国家自然科学基金青年基金资助项目(No.81503104)
广东医科大学大学生创新项目(No.2015ZYDC003
No.2015ZYDC004和2016ZZDC001)
