大鼠盲肠结扎与穿刺脓毒症模型的建立与分析

Establishment and Analysis of Cecum Ligation and Puncture Rat Sepsis Models

脓毒症是重症监护病房(ICU)的一种常见疾病,目前临床上对脓毒症的治疗效率不高。更为严重的是脓毒症患者的死亡率仍然很高,其内部的病理生理过程尚不清楚。许多种类的脓毒症的动物模型已经建立,其中盲肠结扎穿孔(CLP)模型,代表临床脓毒症最理想的模型。然而就CLP模型造模的死亡率、炎症反应以及病理变化而言不同的实验室之间结果有较大差异。目的:为了建立在有限实验条件下稳定性重复性好的大鼠模型,为后续的研究打下了基础。方法:1、确定了实验条件下盲肠结扎程度与大鼠死亡率之间关系。2、收集组织切片,确定其病理改变。3、在相同造模程度下,2种不同麻醉(水合氯醛和异氟烷)分别麻醉的大鼠,观察CLP大鼠死亡率的影响。结果:1、成功确定模型中大鼠盲肠结扎的程度。2、组织病理切片显示脓毒症的典型病理组织损伤,符合进一步研究的需要。3、在研究中发现相同造模程度下异氟醚麻醉组CLP大鼠死亡率为25%;水合氯醛麻醉组CLP大鼠死亡率达68.75%。

Sepsis is a common disease in ICU （intensive care unit）. At present the mortality of sepsis patients is very high and its internal pathophysiology process remains unclear. Many kinds of animal models of sepsis had been established, among these, cecum ligation and puncture （CLP） model is the most ideal model that well representing the clinical sepsis. However, mortalities, immune responses and pathologies of established CLP models varied among different lab groups. Purpose： To establish stable CLP reproducible rat model in our experimental conditions and lay the foundation for subsequent research. Method： 1. Investigating the relationship between the degree of cecum ligation and the rat mortality to select the appropriate level for CLP rat model building. 2. Collecting tissue sections to observe the pathological changes. 3. Comparing the effects of two different anesthetics （chloral hydrate and isoflurane） on the mortality of CLP rats. Results： 1. We found that 50% cecum ligation is suitable for our research. 2.Histopathological biopsy showed typical pathological tissue damages can meet the requirements for further study. 3. We found that even the cecum ligation is in the same degree,the rats＇ mortality was different.the isoflurane anesthesia rats mortality was 25%, however the chloral hydrate anesthesia rats mortality rate was 68. 75%.