摘要
目的:研究microRNA 203(miR-203)对胃癌EIF5A2表达的影响,为进一步阐明miR-203与胃癌的关系提供理论基础。方法:分别应用免疫荧光化学和免疫组织化学检测胃癌细胞和组织中EIF5A2的表达;脂质体miR-203转染干预胃癌细胞后,Real time PCR和Western blot检测细胞中EIF5A2 mRNA和蛋白的表达情况。结果:胃癌细胞株SGC7901胞浆中大量表达EIF5A2蛋白,而细胞核中表达较少;胃癌组织中EIF5A2的表达量明显升高;EIF5A2的表达在脂质体miR-203转染干预胃癌细胞后明显受到抑制。结论:胃癌细胞和组织中存在EIF5A2的表达,且miR-203显著抑制了EIF5A2的表达。
Objective：To study the effect of microRNA 203 （miR-203） on the expression of EIF5A2 in gastric cancer cells,and provide the theoretical basis to the relationship between miR-203 and gastric cancer.Methods：Immunohistochemical and immunofluorescence techniques were used to detect the expression of EIF5A2 in gastric cancer.The mRNA and protein expression of EIF5A2 were detected by Real time PCR and Western blot analysis in gastric cancer cells which were transfected with miR-203 liposome.Results：The EIF5A2 protein was expressed obviously in the cytoplasm of gastric cancer cells,but little in the nucleus.The expression of EIF5A2 was increased significantly in gastric cancer tissues,and the expression of EIF5A2 was inhibited significantly in gastric cancer cells transfected by miR-203 liposome.Conclusion：EIF5A2 was expressed in the gastric cancer cells and tissues,and the expression of EIF5A2 was inhibited significantly by miR-203.
出处
《现代肿瘤医学》
CAS
2017年第22期3587-3590,共4页
Journal of Modern Oncology
基金
陕西省自然科学基金资助项目(编号:2014JM4141)
