CTLA-4基因多态性-318 T/C,CT60 G/A,+49 G/A与结直肠癌易感性的相关分析

Correlation analysis of CTLA-4 gene polymorphisms-318 T/C,CT60 G/A,+49 G/A and susceptibility to colorectal cancer

目的:探讨细胞毒性T淋巴细胞相关抗原-4(cytotoxic T lymphocyte antigen 4,CTLA-4)基因多态性-318 T/C,CT60 G/A,+49 G/A与结直肠癌发生的相关性,为早期预测结直肠癌的发生提供理论依据。方法:自2016年7月至2017年3月,共有167例结直肠癌患者纳入该研究,并选取同期体检健康者245例作为对照。抽取空腹外周静脉血提取DNA后,采用聚合酶链式反应(PCR)技术扩增后直接测序法检测CTLA-4-318 T/C,CT60 G/A,+49 G/A基因位点的多态性,比较两组-318 T/C,CT60 G/A,+49 G/A基因型发生的频率、不同等位基因的分布,分析CTLA-4-318 T/C,CT60 G/A,+49 G/A基因多态性与结直肠癌易感性的关系。结果:与对照组相比,不规律的饮食、排便及肠道基础疾病(息肉、炎症)均可能是结直肠癌患者的高危因素。与携带CTLA-4+49 AA基因型患者相比,携带CTLA-4+49 GG基因型患者发生结直肠癌的危险性为OR=1.698;同时,疾病组+49 G等位基因频率显著高于对照组(OR=0.669,P=0.005)。不同CTLA-4+49 G/A基因型在不同分期结直肠癌患者中的分布具有明显统计学差异(P<0.05)。结论:CTLA-4+49 G/A基因多态性与结直肠癌的发生有关。

Objective：To explore the relationship between cytotoxic T-lymphocyte antigen-4 （CTLA-4） gene polymorphisms -318 T/C,CT60 G/A,＋49 G/A and susceptibility to colorectal cancer,and provide theoretical basis for the early forecasting of colorectal cancer.Methods：The data of 167 cases of colorectal cancer patients were selected as experimetal group,and 245 cases of healthy people were also recruited as control group.Peripheral blood of each participant was collected and genomic DNA was extracted,and then,CTLA-4 -318 T/C,CT60 G/A,＋49 G/A gene polymorphisms were detected by PCR and direct sequencing.The frequences of -318 T/C,CT60 G/A,＋49 G/A genotypes and alleles were compared and the relationship between CTLA-4 -318 T/C,CT60 G/A,＋49 G/A gene polymorphisms and colorectal cancer were analysed.Results：Compared with control group,inregular diet,defecation habits and basic diseases（polyp,inflammation） might be the high risk factors of colorectal cancers.Compared with patients carrying ＋49 AA genotype,the risk of patients carrying ＋49 GG genotype was OR=1.698.Meanwhile,the frequencies of CTLA-4 ＋49 G allele carriers were significantly higher in patients than in controls （OR=0.669,P=0.005）.The distribution of CTLA-4 ＋49 G/A genotpes in different stage of colorectal cancer patients had strong statistical differences（P〈0.05）.Conclusion：CTLA-4 ＋49 G/A genotype had strong correlation with the occurrence of colorectal cancer.