花生中白藜芦醇含量测定的前处理方法优化

Optimization of Pretreatment Methods for Determination of Resveratrol in Peanut

通过比较溶剂直接提取法、高速离心提取法和固相萃取柱提取法3种不同的前处理方法对花生样品进行处理,采用高效液相色谱法(HPLC)对处理后样品中的白藜芦醇含量进行测定,研究并优化花生中白藜芦醇含量测定的前处理方法。结果表明,固相萃取柱提取法效果好,回收率高,该方法的检出限为0.08 mg/kg,加标回收率在96.9%~101.0%,相对标准偏差(RSD)在1.5%~2.1%。

After comparing the recoveries of 3 different sample pretreatment methods, the levels of resveratrol content in samples were detected by High Performance Liquid Chromatography, and studied and optimized the pretreatment method for the determination of resveratrol contents of in peanuts. The results showed that the solid phase extraction method gave the best recovery data, the method detection limit was 0.08 mg/kg, the recovery was 96.9%-101.0% and the relative standard deviation was 1.5%-2.1%.