高效液相法测定黄柏中小檗碱的含量

Determination of Berberine Content in Cortex Phellodendri by High Performance Liquid Chromatography

建立了黄柏中小檗碱含量测定方法。采用高效液相色谱的方法测定黄柏中小檗碱的含量。色谱条件:InertislODS-3色谱柱(250mm×4.60mm,5μm);流动相为乙腈:0.10%磷酸=1:1,流速为0.80m L/min,检测波长为265nm,柱温为30℃;小檗碱的线性范围为1.06μg^11.60μg,其线性曲线为S=4.99×106Q+7.99×105(R2=0.9999),平均加样回收率为98.77%,RSD值为3.87%。该含量方法具有简便、稳定、可重复的特点。

In order to establish a method to determine berberine content in cortex phellodendri, high performance liquid chromatography （HPLC） was adopted to determine the berberine content in cortex phellodendri. The chromatographic conditions were listed as followed： Inertisl ODS-3 column （250mm×4.60mm, 5μm） , the mobile phase acetonitrile ： 0. 10% phosphoric = 1 ： 1, flow rate 0. 80mL/min, detection wave length 265nm for berberline and column temperature 30℃. The results showed that the linear range of berberline was 1.16μg ~ 11.60μg and the linear curve was S = 4. 99× 106Q＋7.99× 105（ R2 = 0. 9999）. The average recovery rate of the method was 98.77%, RSD was 3.87%. The conclusion was that this method was simple, stable and repeatable.