反相离子对HPLC法测定洋金花类生药中的东茛菪碱和阿托品

Determination of Scopolamine and Atropine in Yangjinhua by Reversed-Phase Ion-Pair HPLC

洋金花为茄科曼陀罗属植物白曼陀罗Datura metel等多种植物的花,具平喘止咳、镇痛、解痉功效,其主要成分为东莨菪碱和阿托品。洋金花商品药材中混有多种曼陀罗属植物的花,通过药材的成分分析对药材鉴定是有参考价值的。有关这类成分的分离及含量测定方法较多。

RP Ion-Pair HPLC was applied to isolate and determine the content of scopolamine and atropine from flowers of Datura metel, D. metel cv. ovata, D. arborea, D. innoxia, D. stramonium var. stramonium, D. stramoniun var. tatula, D. stramonium var. inermis. The results indicate that the content of scopolamine in D. metel and the content of atropine in D. arborea and the content of scopolammine in D. stramonium var. tatula are the lowest. Scopolamine is the main content in D. metel, D. metel cv. ovata, D. arborea, while atropine is the dominant in D. innoxia, D. stramonium var. stramonium, D. stramonium var. tatule, D. stramonium var. inermis.