AM真菌对土壤含水量不同条件下甘草生长及甘草酸含量的影响

Effects of AM Fungi on Growth and Glycyrrhizic acid of Glycyrrhiza uralensis under different Soil Relative Water Content

为了探索丛枝菌根(AM)真菌对甘草的侵染特性,揭示土壤相对含水量不同条件下AM真菌对甘草生长及甘草酸含量的影响,以盆栽甘草为研究对象,分别接种摩西管柄囊霉(Funneliformis mosseae)、地表球囊霉(Glomus versiforme)、聚丛球囊霉(G.aggregatum)和幼套球囊霉(G.etunicatum)4种AM真菌,研究了土壤在不同相对含水量条件下AM真菌对甘草的侵染率及侵染后甘草的株高、主根长、地上干重、地下干重及甘草酸含量等,结果表明,接种的4种AM真菌均能与甘草形成丛枝菌根,形成了丰富的丛枝、泡囊结构,侵染率达到70%以上,显著促进了甘草的生长;在50%和65%的土壤相对含水量条件下,AM真菌都对甘草生长有促进作用,在土壤相对含水量小于35%时,甘草生长不良,甘草酸含量低;综合分析土壤在各个不同相对含水量处理中,接种摩西管柄囊霉的甘草适应生长的范围更大,甘草酸含量更高,综合来看,摩西管柄囊霉更有利于提高甘草对干旱的适应性,促进甘草生长效果显著,并且在干旱条件下仍保持较高的甘草酸含量。

In order to explore the infection characteristics of arbuscular mycorrhiza fungi against Glycyrrhiza uralensis and learn the relationship between arbuscular mycorrhiza and growth of G.uralensis and yield of glycyrrhizic acid under different relative water content of soil,potted G.uralensis was taken as the research object to be inoculated with Glomus mosseae,G.versiforme,G.aggregatum and G.Etunicatum separately.By assaying the infection rate of arbuscular mycorrhizal fungi against G.uralensis,stem length,main root length,overground weight,underground weight and content of glycyrrhizic acid after infection,the influence of arbuscular mycorrhiza on the growth and metabolism of G.uralensis under different relative water content of soil was studied.The result showed that the four kinds of arbuscular mycorrhizal fungi inoculated all could form arbuscular mycorrhiza with G.uralensis and abundant arbuscular and vesicle structure was formed with the infection rate as high as above 70%,significantly facilitating the growth of G.uralensis.Under 50%and 65% relative water content of soil,the arbuscular mycorrhiza could hasten G.uralensis growth.When the relative water content of soil was less than 35%,G.uralensis could not grow normally.By comprehensive analysis under different relative water content of soil,the G.uralensis inoculated with G.mosseae showed better growth and contained much glycyrrhizic acid.In general,G.mosseae is more conducive to improving the adaptability of G.uralensis and generates greater growth effect.