AANAT基因编码区多态性与家兔生长性状的关联分析

Association Analysis between Polymorphism of Coding Sequence of AANAT Gene and Growth Traits of Rabbits

为研究五羟色胺-N-乙酰基转移酶(AANAT)基因编码区(CDS区)多态性和家兔生长性状的关联性,以天府黑兔、伊拉兔和新西兰兔作为实验材料,对AANAT基因的外显子进行PCR扩增,直接测序检测家兔AANAT基因外显子的单核苷酸多态性。结果表明:在3个兔品种的CDS区上均发现有5个完全连锁突变位点,分别是C.132T>C、C.153T>C、C.159 A>T、C.234 A>G、C.291 A>G,形成了野生型TTTTAAAAAA、杂合型TCTCATAGAG、突变型CCCCTTGGGG 3种基因型,突变型新西兰兔在56、70、84日龄的体重显著高于野生型和杂合型(P<0.05),而突变型伊拉兔和天府黑兔在70、84日龄的体重显著高于杂合型和野生型(P<0.05)。综上可知,AANAT基因可作为肉兔生长性状的候选基因。

To study the association between Coding sequence（CDS）of five HT -N- acetyltransferase gene （ AANAT ） polymorphism and growth traits in rabbits. In the study we used direct sequencing method to detect single nucleotide polymorphism of exons of AANAT gene region with Tianfu black rabbit, IRA rabbit and New Zealand rabbit as experimental materials. There were 5 complete linkage mutation sites（C.132 T〉C, C.153T〉C, C. 159 A〉T, C.234 A〉G, C.291A〉G） in the CDS ofAANAT gene in the three rabbit breeds. 3 genetypes（wild type： TTTTAAAAAA, heterozygous： TCTCATAGAG mutant type： CCCCTTGGGG ） were formed. In New Zealand, the 56, 70 and 84 days body weight of mutant type was significantly higher than those of other types rabbits and in the IRA rabbit and Tianfu black rabbit （P〈0.05）,70 and 84 days body weight were higher （P〈0.05）. The AANATgene can be used as a candidate gene for growth traits in rabbit.