白介素-8在原发性痛风性关节炎患者外周血的表达及意义

Expression and significance of IL-8 in patients with primary gouty arthritis

目的分析白介素-8(IL-8)在痛风性关节炎(GA)患者外周血中的表达变化,探讨其临床意义。方法选择急性痛风性关节炎(AGA组)、非急性痛风性关节炎间歇期(NAGA组)和健康体检者(HC组)各43例。利用RTPCR和Western blot方法分别检测外周血单个核细胞(PBMCs)中IL-8mRNA和蛋白表达,再用ELISA法检测各组血浆IL-8水平。结果 IL-8mRNA在AGA组PBMCs中的表达高于NAGA组和HC组(均P<0.01),在NAGA组的表达高于HC组(P<0.01)。PBMCs中的IL-8蛋白水平在AGA组高于NAGA组和HC组(均P<0.01),且HC组最低,但NAGA组和HC组比较差异无统计学意义(P>0.05)。血浆中IL-8水平在AGA组和NAGA组显著高于HC组(均P<0.01),NAGA组显著高于HC组(P<0.05)。结论 IL-8在GA患者的外周血中异常表达,提示IL-8在GA的病理机制中可能发挥重要的作用。

Objective To analyze the expression and clinical significance of IL-8 in peripheral blood in patients with gouty arthritis （GA）. Methods The peripheral blood were collected from 43 patients with acute gouty arthritis （AGA）, 43 patients with non-acute gouty arthritis （NAGA） and 43 healthy controls （HC）. The expression levels of IL-8 mRNA and protein in peripheral blood mononuclear ceils （PBMCs） were detected by real-time fluorescent quantitative polymerase chain reaction and Western blot. The protien expressions of tL-8 were measured by enzyme linked immunosorbent assay （ELISA） in plasma. Results The expression of IL-8 mRNA in PBMCs of the AGA group was significantly higher than that of the IGA and HC groups （all P〈0.01）, and IGA group higher than HC groups （P〈0.01）. Compared with IGA and HC groups, the protein expression of IL-8 significantly increased in PBMCs of the AGA group, and the HC group was the lowest. There were no statistical differences between IGA and HC groups. The expression of IL-8 was signifi- cantly higher in plasma of the AGA group compared with the IGA and HC groups （all P〈0.01）, and IGA group signifi- cantly higher than HC groups （P〈0.05）. Conelusion The expression of IL-8 changes significantly in GA patients in comparison with HC, which implies that IL-8 may have an important role in progression of GA.