心肌细胞缺氧/复氧损伤下硫辛酸的保护性作用研究

Protective effects of lipoic acid on cardiomyocytes undergoing hypoxia/reoxygenation injury

目的 研究硫辛酸对H9c2心肌细胞缺氧/复氧损伤模型的保护作用,探讨其相关机制.方法 将8株H9c2大鼠心肌细胞株进行培养,待细胞生长至满视野时传代后分为常氧组、缺氧/复氧组和硫辛酸组.检测三组H9c2心肌细胞存活率、乳酸脱氢酶（LDH）活性、丙二醛（MDA）和血红素氧合酶-1（HO-1）,并进行比较.结果 缺氧/复氧组和硫辛酸组H9c2心肌细胞存活率明显低于常氧组[（52.86±6.39）％、（69.25±7.63）％比（92.31±7.82）％],但硫辛酸组明显高于缺氧/复氧组,差异均有统计学意义（P〈0.01）.缺氧/复氧组和硫辛酸组LDH活性和MDA明显高于常氧组[（286.37±27.49）、（209.72±25.63）U/L比（126.32±18.94）U/L和（1.72±0.06）、（1.13±0.07）μmol/L比（0.68±0.06）μmol/L],但硫辛酸组明显低于缺氧/复氧组,差异均有统计学意义（P〈0.01）.缺氧/复氧组和硫辛酸组HO-1明显高于常氧组[（213.71±18.94）％、（367.26±23.07）％比（87.92±19.23）％],而且硫辛酸组明显高于缺氧/复氧组,差异均有统计学意义（P〈0.01）.结论 硫辛酸对心肌细胞缺氧/复氧损伤具有保护作用,增加HO-1含量,发挥其抗氧化应激的作用.

Objective To study the protective effect of lipoic acid （LA） on H9c2 cardiomyocytes hypoxia/reoxygenation injury model, and explore its relevant mechanism. Methods Eight strains of H9c2 cardiomyocytes, passaged after cultured to a full view, were divided into 3 groups：normoxia group, hypoxia/reoxygenation group and LA group. The cell survival rate, lactate dehydrogenase （LDH） activity, malondialdehyde （MDA） and heme oxygenase-1 （HO-1） levels were detected and compared. Results The cell survival rates of H9c2 cardiomyocytes in hypoxia/reoxygenation group and LA group were significantly lower than those in normoxia group：（52.86 ± 6.39）％, （69.25 ± 7.63）％vs. （92.31 ± 7.82）％, while the cell survival rate of H9c2 cardiomyocytes in LA group was significantly higher than that in hypoxia/reoxygenation group, and there were statistical differences （P〈0.01）. The LDH activity and MDA in hypoxia/reoxygenation group and LA group were significantly higher than those in normoxia group：（286.37 ± 27.49）, （209.72 ± 25.63） U/L vs. （126.32 ± 18.94） U/L, and （1.72 ± 0.06）, （1.13 ± 0.07）μmol/L vs. （0.68 ± 0.06） μmol/L, while those data in LA group were significantly lower than those in hypoxia/reoxygenation group, and there were statistical differences （P〈0.01）. The HO-1 in hypoxia/reoxygenation group and LA group were significantly higher than that in normoxia group：（213.71 ± 18.94）％, （367.26 ± 23.07）％vs. （87.92 ± 19.23）％, and HO-1 in LA group was significantly higher than that in hypoxia/reoxygenation group, and there were statistical differences （P〈0.01）. Conclusions The LA plays a protective role on myocardial cell with hypoxia/reoxygenation injurythough increasing the level of HO-1 against oxidative stress.