摘要
Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods： The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group（distant acupoints ＋ local acupoints）, EA 2 group（local acupoints）, and EA 3 group（distant acupoints）. Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results： Compared with the model group, thermal withdrawal latencies increased after EA treatment（P〈0.01）. The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery（P〈0.05）. Compared with the model group, EA stimulation（EA 1 and EA 2 groups） reversed the up-regulation of COX 2 m RNA expression（P〈0.05）. EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions： EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of ＂distant acupoints ＋ local acupoints＂, and ＂local acupoints＂ intervention may be superior to that of ＂distant acupoints＂ intervention. Microglia may be related to the formation of COX 2.
Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods： The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group（distant acupoints ＋ local acupoints）, EA 2 group（local acupoints）, and EA 3 group（distant acupoints）. Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results： Compared with the model group, thermal withdrawal latencies increased after EA treatment（P〈0.01）. The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery（P〈0.05）. Compared with the model group, EA stimulation（EA 1 and EA 2 groups） reversed the up-regulation of COX 2 m RNA expression（P〈0.05）. EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions： EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of ＂distant acupoints ＋ local acupoints＂, and ＂local acupoints＂ intervention may be superior to that of ＂distant acupoints＂ intervention. Microglia may be related to the formation of COX 2.
基金
Supported by the Project of Beijing University of Chinese Medicine,China(No.JYB22-JS022)
