高迁移率族蛋白B1和晚期糖基化终末产物受体在胃癌组织中的表达及意义

Expression and clinical significance of HMGB1 and RAGE protein in gastric cancer tissues

目的探讨胃癌组织中高迁移率族蛋白B1(HMGB1)和晚期糖基化终末产物受体(RAGE)蛋白的表达及临床意义。方法选取2013年6月至2016年6月间青岛市胶州中心医院收治的230例胃癌患者,均行手术治疗,取胃癌组织作为观察组,取癌旁正常组织作为对照组。检测两组组织内HMGB1和RAGE的表达情况,观察其与临床病理参数的关系,采用Spearman相关分析法分析HMGB1和RAGE的相关性。结果观察组中HMGB1和RAGE阳性表达率分别为43.9%(101/230)和44.8%(103/230),对照组中HMGB1和RAGE阳性表达率分别为17.4%(40/230)和19.6%(45/230),两组比较,差异有统计学意义(P<0.05)。HMGB1在不同TNM分期和有无淋巴结转移患者间的表达比较,差异有统计学意义(P<0.05),RAGE与有无淋巴结转移患者间的表达比较,差异有统计学意义(P<0.05)。Spearman相关分析显示,HMGB1和RAGE表达呈正相关关系。结论胃癌组织中的HMGB1和RAGE会呈高表达,其对胃癌转移和浸润有预测作用,且HMGB1和RAGE存在正相关关系。

Objective To explore the expression and clinical significance of high mobility group protein B1（ HMGB1） and receptor for advanced glycation end products（ RAGE） protein in gastric cancer tissues. Methods A total of 230 patients with gastric cancer treated at Jiaozhou Central Hospital of Qingdao from June 2013 to June 2016 were selected. All patients underwent surgical treatment. The gastric cancer tissues were collected as the research group and the normal tissues adjacent to the tumors were collected as the control group. The expression of HMGB1 and RAGE were detected in the two groups. The relationship between the clinical pathological parameters and the expression of HMGB1 and RAGE were observed. The correlation between HMGB1 and RAGE was analyzed by using Spearman＇s correlation method. Results The positive expression rates of HMGB1 and RAGE were 43. 9%（ 101/230） and 44. 8%（ 103/230） for the study group and 17. 4%（ 40/230） and 19. 6%（ 45/230） for the control group（ P〈0. 05）. There were statistically significant differences in the expression of HMGB1 among patients with different TNM stages and with or without lymph node metastases. There were statistically significant differences in the expression of RAGE between patients with and without lymph node metastases（ all P〈0. 05）. Spearman correlation analysis showed that HMGB1 and RAGE expression was positively correlated（ P〈0. 05）. Conclusion High expression of HMGB1 and RAGE in gastric cancer tissues is observed,which could predict the metastasis and invasion of gastric cancer,and there is a positive correlation between HMGB1 and RAGE.