摘要
The conformation of silk fibroin （SF） frozen with polyethylene glycol （PEG） at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG：SF from 1：5 to 10：1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at -20 ℃, and greatly depended on the cooling rate, PEG：SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.
The conformation of silk fibroin （SF） frozen with polyethylene glycol （PEG） at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG：SF from 1：5 to 10：1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at -20 ℃, and greatly depended on the cooling rate, PEG：SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.
基金
financially supported by the National Natural Science Foundation of China(No.21374022)
