银杏叶提取液抗肢体缺血再灌注损伤

The effect of Ginkgo Biloba Succi in combating platelet mediated injury of limb during ischemia and reperfusion

目的 观察银杏叶提取液对肢体缺血再灌注损伤的影响。方法 制作兔肢体缺血再灌注损伤动物模型 ,并分为对照组、再灌注组和治疗组。检测不同时相点血小板计数、血栓素B2 (TXB2 )和 6 酮 前列腺素F1α(6 Keto PGF1α)含量 ,测定骨骼肌丙二醛 (MDA)、线粒体钙 (MitochondrialCa)含量和组织湿 /干重量比值 (Wet/Dry)。结果 再灌注组与对照组比较 ,以上各项指标变化明显。银杏叶提取液可抑制血小板活化、降低血浆TXB2 和 6 Keto PGF1α水平 ,减少骨骼肌MDA、线粒体Ca含量和组织湿 /干重量比值。治疗组各项测定指标较再灌注组有比较明显改善。结论 银杏叶提取液对肢体缺血再灌注损伤骨骼肌有保护作用。

Objective To observe the effect of Ginkgo Biloba Succi on ischemia and reperfusion injury of the limbs Methods The experimental models of ischemia and reperfusion injury of limb was produced in the rabbits which were divided into control group,reperfusion group and treatment group.The platelet count,TXB 2 and 6 Keto PGF 1α in plasma were assayed at different phase.The MDA,Mitochondrial Ca content and Wet/Dry ratio of skeletal muscle were measured respectively.Results The index of reperfusion group changed significantly as compared with those of control group.Ginkgo Biloba Succi inhibited the platelet activation,reduced the level of TXB 2 and 6 Keto PGF 1α in plasma and decreased the MDA,Mitochondrial Ca content and Wet/Dry ratio of skeletal muscle.The index of treatment group improved significantly as compared with those of reperfusion group.Conclusion Ginkgo Biloba Succi might have the protective effect on muscle cells during ischemia and reperfusion injury of limb.