香菇多糖对肝癌细胞Huh7.5.1增殖和侵袭的影响

The Effects of Lentinan on the pr oliferation and Invasion of Huh7.5.1

目的:探讨香菇多糖对肝癌细胞Huh7.5.1增殖和侵袭的影响。方法:采用MTT法检测6.25、12.5、25、50、100μg/m L香菇多糖分别作用肝癌细胞24、48、72 h后,肝癌细胞增殖抑制率变化;采用Transwell侵袭实验考察其对细胞侵袭的影响。结果:6.25、12.5、25、50、100μg/m L香菇多糖分别作用肝癌细胞24、48、72h后,细胞的抑制率呈浓度和时间依赖性升高,100μg/m L香菇多糖作用48 h后肝癌细胞抑制率为(50.36±5.13)%,作为后续实验浓度和作用时间;100μg/m L香菇多糖处理肝癌细胞48 h后,肝癌Hep G2细胞体外侵袭能力明显抑制(P<0.05)。结论:香菇多糖能够抑制肝癌细胞增殖和侵袭。

Objective： To investigate the effects of Lentinan on the proliferation and invasion of hepatocellular car- cinoma cell line Huh7.5.1. Methods： MTT assay was used to detect the proliferation of hepatocellular carcinoma ceils at 24, 48 and 72 h after 6.25, 12, 25 and 50 μLg of 100 μL g/ml lentinan. The proliferation of hepatocellular carcinoma cells was observed by Transwell invasion assay. Results： After 24, 48 and 72 h of lentinan cells were treated with 6.25, 12.5, 25, 50 and 100 μg/ml, the inhibition rate of the ceils was increased in a concentration and time-dependent manner. After 100 h/ml lentinan （P〈0.05）. The inhibitory rate of HepG2 cells was significantly higher than that of the control group （P〈0.05）. The inhibitory rate of HepG2 cells was significantly higher than that of the control group （P〈0.05）. The inhibitory rate of hepatocellular carcinoma cells was 50.36 ± 5.13% Conclusion： Lentinan can inhibit the proliferation and invasion of hepatocarcinoma cells.