蛭龙活血通瘀胶囊对脑出血大鼠神经功能及脑水肿改善作用随机平行对照研究

The Random Parallel Control Study of Zhilonghuoxuetongyu Capsule Improve Nerve Function and Brain Edema in Cerebral Hemorrhage Rats

[目的]观察蛭龙活血通瘀胶囊对脑出血大鼠神经功能及脑水肿的改善作用。[方法]将120只大鼠随机分为假手术组、模型组、蛭龙活血通瘀胶囊高、中、低剂量组,各组又分为12h、48h、72h三个亚组,采用自体尾部血注入法制备大鼠脑出血模型,各组进行神经行为学检测(NSS评分),HE染色光镜下观察脑组织形态学改变,检测脑组织含水量及脑系数。[结果]与模型组比较,蛭龙活血通瘀低剂量组术后72h,中剂量组术后48h、72h及高剂量组术后12h、48h、72h NSS评分具有显著差异(P<0.01);蛭龙活血通瘀低、中、高剂量组脑含水量均明显降低,差异具有统计学意义,低剂量组12h(P<0.05),其余各时间点各药物组(P<0.01);在48h、72h时各药物组脑系数均具有显著性差异(P<0.01)。[结论]蛭龙活血通瘀胶囊通过对脑出血大鼠脑水肿程度的改善起到明显的脑保护作用,该作用存在一定的量效关系,高剂量组的作用最为明显,脑保护的作用也最强。

[Objective] To observe the improvement of nerve function and brain edema in cerebral hemorrhage rats by Zhilonghuoxuetongyu capsule. [Methods] 120 rats were randomly divided into control group, model group, Zhilonghuoxuetongyu capsule high, medium and low dose group, each group is divided into 12h, 48h, 72h three subgroups, autologous blood tail injection prepared hemorrhage rats model, each neuroethology test （NSS score）, HE staining were observed under light on brain morphology change, testing the brain water content and cerebral coefficient.[Results] Compared with model group, Zhilonghuoxuetongyu capsule low dose group of 72h, the medium group 48h, 72h and high dose group 12h, 48h, 72h after surgery, NSS scores have significant difference （/9 〈0.01）; Zhilonghuoxuetongyu capsule low, medium and high dose group of brain water content were significantly reduced, have statistically significant differences, 12h in the lower dosage treatment groups （P〈0.05）, the others each point in time each drug group （P〈0.01）; At 48 h, 72h brain coefficient of each drag group were with significant difference （P〈0.01）. [Conclusion] Zhilonghuoxuetongyu capsule on the improvement of the degree of edema of cerebral hemorrhage rats by a clear brain protection, the role has certain concentration-response relationship, the effect of high dose group is most obvious, the effect of brain protection is strongest.