多囊卵巢综合征外周血miRNA-93的表达及作用意义

Clinical significance of circulating mi RNA-93 levels in patients with polycystic ovary syndrome

目的探究外周血miRNA-93在PCOS患者的表达情况及临床意义。方法选取本院妇产科就诊的40例PCOS患者为观察组,同时选取50例非PCOS患者为对照组。采用己糖激酶法检测各血清标本中空腹血糖(Fasting blood sugar,FBS);放射免疫法测定总睾酮(Total testosterone,TT)、雄烯二酮(androstenedione)和性激素结合球蛋白(SHBG,sex hormone-binding globulin)的水平,生化分析仪检测血清超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)的水平;电化学发光法测定空腹血清胰岛素(fasting serum insulin,FSI);计算胰岛素抵抗指数(HOMA-IR);QRT-PCR检测外周血miRNA-93的表达水平。结果观察组患者血清FSI和TT激素指标均明显升高(P<0.05),且HOMA-IR值也显著高于对照组(P<0.05);观察组血清miRNA-93水平显著高于对照组(P<0.05);PCOS患者血清miRNA-93与胰岛素、HOMA-IR和TT指标均没有明显的相关性;PCOS患者的miRNA-93最佳cutoff值为1.035,其中敏感性70.0%,特异性67.5%,AUC值0.704。结论 PCOS患者血清miRNA-93的表达显著高于对照组,对于PCOS患者miRNA-93的检测将有助于临床上对该疾病的诊断和治疗效果。

Objective：To investigate and detect circulating miRNA-93 levels in patients with PCOS. Methods：A total of 40 PCOS patients in our hospital as the observed group and 40 without PCOS women as control group were studied in this research. Serum levels of fasting blood sugar（FBS）was measured by hexokinase method;Total testosterone（TT）,androstenedione and sex hormone-binding globulin（SHBG）were detection via radioimmunoassay（RIA）;High-sensitivity C-reactive protein（hs-CRP）were measured through biochemical analyzer;Fasting serum insulin（FSI）was detected by electrochemiluminescence;HOMA-IR were calculated in all patients. The expression of serum miRNA-93 were measured by QRT-PCR. Results：Serum FSI and TT levels were significantly higher in observed group than those in control group（P〈0.05）. Besides,compared with control group,serum miRNA-93 levels were significantly higher in observed group and there was significant difference between the two groups（P〈0.05）. Furthermore,there was not positive correlation between miRNA-93 and HOMA-IR/TT/FSI. The best cutoff value for serum miRNA-93 concentration to diagnose PCOS was 1.035（sensitivity 70.0%,specificity 67.5%,and AUC 0.704）. Conclusion：Patients with PCOS have higher serum miRNA-93 levels and it is thought to be effective on PCOS diagnosis and treatment effect.