摘要
目的探讨双孔钾离子通道TASK-1、TRAAK蛋白在精子中的表达及其在弱精子症发生机制中的作用。方法非连续密度梯度离心分离、纯化正常男性和弱精子症患者精子后,采用Western印迹技术从蛋白水平检测TASK-1、TRAAK的表达及差异。结果 TASK-1、TRAAK蛋白在正常男性和弱精子症患者精子中均有表达;其中,TASK-1蛋白在弱精子症患者精子中的表达量与正常男性没有明显差异(P>0.05),TRAAK蛋白在弱精子症患者精子中的表达明显低于常男性(P<0.05),这与我们前期的RT-PCR结果一致。结论 TRAAK在弱精子症患者精子中的表达下调可能是精子活力下降的原因之一。
Objective：To investigate the expression of K2 P channel TASK-1、TRAAK protein in the sperm and the role of them in the pathogenesis of asthenozoospermia. Methods：Semen samples from 23 normal male persons and 35 asthenozoospermia patients were separated and purified by Percoll discontinuous density gradients and the expressions of TASK-1、TRAAK protein were detected by Western blot. Results：TASK-1,TRAAK protein were expressed in the sperm both in the normal male persons and the asthenozoospermia patients;the expression of TRAAK protein was significantly lower in the sperm of the asthenozoospermia patients than in those of the normal male persons（P〈0.05）,while,there was no significant difference of TASK-1 protein in the two groups（P〉0.05）,the results are consistent with our previous RT-PCR experiment. Conclusion：The expression of TRAAK is significantly decreased in the sperm of asthenozoospermia patients,which might be one of the causes of Sperm vitality decline.
出处
《中国优生与遗传杂志》
2017年第9期112-114,共3页
Chinese Journal of Birth Health & Heredity
基金
湖北省卫生计生科研项目基金资助(WJ2015MB254)
