陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究

Analysis of the Correlation between Pigment Glands and (-)/(+)-Gossypol Contents in Upland Cotton

【目的】研究不同基因型棉花不同器官的色素腺体与棉酚旋光体含量之间的相关性。【方法】以6个陆地棉材料中棉所12、中棉所12(无)、珂字棉312、珂字棉312(无)、IF11和冀棉14为对象,精确测定主要器官的色素腺体密度和大小,并采用柱前衍生拆旋方法进行高效液相色谱分析,测定其相应的棉酚旋光体含量,然后对色素腺体和棉酚旋光体含量进行相关性分析。【结果】低酚棉全株无色素腺体,但在各器官中仍能检测到低于0.02%(质量分数)的少量游离棉酚存在。有酚棉各器官均具有典型的色素腺体,其中棉仁的色素腺体最密,苞叶最稀;色素腺体大小(直径)以铃壳最大,花瓣和棉仁最小。不同器官中,总棉酚含量以棉仁最高,苞叶最小;棉仁和花瓣的右旋棉酚含量大于左旋棉酚,而萼片、铃壳、上部叶、中部叶和下部叶的左旋棉酚含量大于右旋棉酚。有酚棉的色素腺体密度与大小之间呈极显著负相关;而总棉酚含量与不同棉酚旋光体含量之间,以及左右旋棉酚含量之间呈极显著正相关。色素腺体密度与总棉酚含量以及不同棉酚旋光体含量之间呈极显著的正相关,而色素腺体大小与右旋棉酚含量之间呈显著的负相关。【结论】棉仁与花瓣的总棉酚含量存在高度的相关性,因此,可以通过花瓣的棉酚含量来判断棉仁的棉酚含量。

b[Objective] The aim of this study was to examine the correlation between pigment glands and（-）/（＋）-gossypol contents in different organs of different upland cotton genotypes. [Method] Using upland cotton materials CCRI 12, CCRI 12（glandless）,Coker 312, Coker 312（glandless）, IF11 and Jimian 14, we accurately measured the density and size of the pigment glands, tested（-）/（＋）-gossypol contents by pre-column derivatization-high performance liquid chromatography（HPLC）, and then analyzed the correlation between pigment glands and（-）/（＋）-gossypol contents. [Result] The whole plants of low gossypol cotton lines were without pigment glands, but free gossypol could still be detected in different organs at mass fractions less than 0.02%. The organs of glanded cotton all had typical pigment glands, the gland density was highest in the seed and lowest in the bract. The gland size（diameter） was largest in the boll shell and smallest in the petal and seed. Across different organs, the total gossypol content was highest in the seed and lowest in the bract. The contents of（＋）-gossypol in the seed and petal were higher than（-）-gossypol. Conversely, the（-）-gossypol contents of the calyx, boll shell, upper leaf, middle leaf, and lower leaf were higher than（＋）-gossypol. There was a significant negative correlation between the density and size of glands in glanded cotton. There were significant positive correlations between total gossypol content and（-）/（＋）-gossypol contents, and between（＋）-gossypol content and（-）-gossypol content. Gland density was positively correlated with total gossypol content and（-）/（＋）-gossypol contents.Gland size was negatively correlated with（＋）-gossypol content, and showed no significant correlation with（-）-gossypol content or total gossypol content. [Conclusion] Overall, the strong positive correlation between total gossypol content in the seed and petal suggests that we can use the petal gossypol content to infer the seed gossypol content.