核糖霉素薄层扫描含量测定

Determination of Ribostamycin and Neamine by TLC

核糖霉素是80年代开始使用的氨基糖甙类抗生素,由于耳毒性较氨基糖甙类抗生素低,尤其适合儿童使用,故对其质量控制很有实用价值。由于生产工艺等因素,核糖霉素产品中除主成分外,还含有结构相似而活性很低的杂质。从本实验薄层色谱图上看,核糖霉素产品中至少存在三种杂质,其一为新霉胺,另外两种结构待定。新霉胺既是生产过程中的副产品。

A thin layer chromatographic method was described for determination of ribostamycin and neamine in ribostamycin sample. Standard substance ribostamycin was prepared by TLC. They were separated on silica gel-CMC plates with chloroform-methanol-ammonia water-aceton (2: 3: 2: 2). Spots were visulized with 0.03% ninhydrin, and determined by using CS-930 dual wavelength TLC scanner at λ_S=535 nm, λ_R=700 nm. Six samples were determined.