千斤拔多糖对小鼠免疫功能的调节作用

Effects of Flemingia polysaccharide on modulating immune function in mice

为研究千斤拔多糖对正常及免疫低下小鼠免疫功能的调节作用,该研究选用SPF级BALB/c小鼠,免疫抑制小鼠采用隔天皮下注射环磷酰胺(40 mg·kg^(-1))5次,通过测定脾脏、胸腺质量及计算脏器指数,采用碳粒廓清法计算单核巨噬细胞吞噬功能,在鸡红细胞免疫后测定小鼠血清溶血素抗体水平,同时观察高、低剂量千斤拔多糖(剂量分别为500、1 000 kg·d^(-1))对正常小鼠及免疫低下小鼠免疫调节的影响。结果表明:千斤拔多糖高、低剂量组对正常及免疫低下小鼠均有不同程度的增加脾脏指数、胸腺指数的作用;千斤拔多糖高、低剂量组对正常及免疫低下小鼠均能提高廓清指数,但没有统计学意义;千斤拔多糖高剂量组对正常及免疫低下小鼠的血清溶血素抗体水平都有显著性提高。这说明千斤拔多糖能有效提高正常及免疫低下小鼠免疫功能的作用。

To study the immunoregulatory activity of Flemingia polysaccharide on immuno compromised by cyclophosphamide and normal mice. We measured thymus,spleen and calculated the visceral index; measured mononuclear-mac-rophage phagocytic function by the Carbon Grain Kuo Clear Law; measured the level of serum hemolysin after erythrocyte immune,to observe the effect of the high and low dose of Flemingia polysaccharide（ doses of 500,1 000 kg·d^-1） on the normal SPF BALB/c mice. We injected cyclophosphamide（ 40 mg·kg^-1） five times subcutaneously every other day to lead to the immunosuppression mice. We measured thymus,spleen and calculated the visceral index; measured mononuclear-macrophage phagocytic function by the Carbon Grain Kuo Clear Law; measured the level of serum hemolysin after erythrocyte immune,observe the effect of the high and low dose of Flemingia polysaccharide on the immunocompromised SPF BALB/c mice. High and low dose of Flemingia polysaccharide could increase index of immune organs,carbon clearance capacity in varying degress（ P0.05 or P0.01）,and could also enhance the clearance index on normal and immunocompromised mice,but it have no significance,and could increase antibody of serum hemolysin significantly（ P0.05 or P0.01） as well. These indicate that Flemingia polysaccharide can obviously enhance the immune function in normal and immuno compromised mice.