摘要
目的:对"砍伤法"所结人工沉香进行质量评价,为其科学种植与采摘提供参考。方法:采用气相色谱-质谱联用法和高效液相色谱法,对3批"砍伤法"所结人工沉香(编号为1、2、3号,分别结香5、10、20年)的挥发性成分、液相特征图谱、沉香四醇和醇溶性浸出物含量进行检测。结果:3批人工沉香的挥发性成分主要为芳香族类化合物、倍半萜类化合物、脂肪酸类化合物和色酮类化合物;2、3号样品的液相特征图谱与沉香对照品基本一致;3批样品的沉香四醇含量为0.078%~0.254%,醇溶性浸出物含量为12.4%~20.8%。1号样品的液相特征图谱与沉香四醇含量不符合2015年版《中国药典》(一部)相关规定。结论:"砍伤法"所结人工沉香与天然沉香具有相似的挥发性化学成分,结香10年以上的人工沉香质量符合2015年版《中国药典》(一部)中"沉香"项下的规定,可以代替天然沉香入药。
OBJECTIVE:To conduct the quality assessment of artificial Aquilaria sinensis by"cutting inducing technique",and provide reference for its scientific planting and harvest. METHODS:GC-MC and HPLC were adopted to detect the volatile ingredients,characteristic spectrum,incense tetraol and alcohol-soluble extract contents in 3 batches of artificial A. sinensis(Num. 1,2,3,respectively for 5,10,20 years)by"cutting inducing technique". RESULTS:The volatile ingredients of 3 batches of artificial A. sinensis mainly consisted of aromatic compounds,sesquiterpene compounds,fatty acid compounds and chromone compounds. The characteristic spectrums of samples 2,3 were basically the same with the reference substance of A. sinensis. The incense tetraol contents of 3 batches of samples were 0.078%-0.254%,and alcohol-soluble extract contents were 12.4%-20.8%. The characteristic spectrum and the incense tetraol content of sample 1 were not conformed to the standards in Chinese Pharmacopoeia(2015 edition,part 1). CONCLUSIONS:Artificial A. sinensis by"cutting inducing technique"shows similar volatile ingredients to natural A. sinensis. The quality of artificial A. sinensis for more than 10 years is conformed to the standards in Chinese Pharmacopoeia(2015 edition,part 1),which can be used as medicine,replacing the natural A. sinensis.
出处
《中国药房》
CAS
北大核心
2017年第28期3996-3999,共4页
China Pharmacy
基金
中山市科技计划项目(No.2014A2FC241)