橙皮素对异丙基肾上腺素诱导大鼠心衰的保护作用

Cardioprotective Effects of Hesperetin Against ISO-induced Heart Failure in Rats

目的观察橙皮素对大剂量异丙基肾上腺素(ISO)诱导大鼠心衰的保护作用,并探讨其机制。方法将SD大鼠随机分为正常(C)组、橙皮素对照(H)组、异丙基肾上腺素(I)组和橙皮素治疗+I(T)组,I组和T组接受皮下注射大剂量ISO(150 mg/kg),2周后行超声心动图检测。确定造模成功后,H组及T组给予橙皮素(100mg/kg)灌胃,I组给予等量的溶剂乙醇,连续给药10d后测定各组大鼠心脏功能包括左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室射血分数(EF)、左室缩短分数(FS)变化及血清丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(TAOC)的变化,观察各组大鼠心肌的病理变化。结果与C组比较,I组LVEDD变化不大,LVESD值增大、EF、FS降低,氧化应激指标中MDA值升高,SOD、TAOC降低,各指标差异有统计学意义(P<0.05);与I组比较,T组大鼠LVEDD无明显变化,LVESD降低,EF、FS升高,血清MDA值降低,SOD、TAOC升高,各指标差异有统计学意义(P<0.05)。H组各指标较C组无明显变化。结论橙皮素可能通过减轻氧化应激,降低MDA,升高SOD、TAOC,从而改善心衰大鼠的心脏功能。

Objective To explore the protective effects and possible mechanisms of hesperetin against isoproterenol（ISO）-induced heart failure（HF）in rats.Methods Forty Sprague-Dawley（SD）rats were randomly divided into four groups：control group（group C）,hesperetin group（group H）,isoproterenol group（group I）and H＋I group（group T）.Rats in group I and group T were subcutaneously injected with ISO（150 mg/kg）once a day for 2 consecutive days.The model was successfully established in heart failure was determined by echocardiography after 2 weeks.Hesperetin was administered at the dosage of 100 mg/kg,po by gavage for a period of 10 days in group H and group T.Cardiac function of all rats including left ventricular end-diastolic diameter（LVEDD）,left ventricular end-systolic dimension（LVESD）,left ventricular ejection fractions（EF）,left ventricular fractional shortening（FS）was assessed by echocardiography.Collecting serum of all rats,malondialdehyde（MDA）,superoxide dismutase（SOD）,total antioxidant capacity（TAOC）were detected by thiobarbiturieacid colorimetry,WST-1 method and colorimetry method respectively.ResultsThere was not obvious changes of cardiac function in group C and group H.ISO inhibited the cardiac function of rats significantly including LVESD,EF,as well as FS,compared to group C（P 0.05）.LVESD was increased while EF and FS were decreased.The level of MDA in group I was significantly higher（P 0.05）while the levels of SOD and TAOC were significantly lower than group C（P 0.05）.Hesperetin attenuated the effect of heart failure induced by ISO.Cardiac function of rats including LVESD,EF,as well as FS was improved in group T compared to group I（P 0.05）.LVESD was declined,EF and FS were ascended.The level of MDA was significantly lower,the levels of SOD,TAOC in group T were significantly higher than that in group I（P 0.05）.ConclusionHesperetin can attenuate ISO-induced heart failure in rats.Its possible mechanisms may be associated with regulating oxidative stress.