用于基因水平转移的微流控方法研究

A microfluidic method applied to research on gene transfer

为实现对于基因转移过程的实时和原位观测,设计了1种实验方法。利用接种有供体菌和受体菌的微流控芯片作为实验载体,并通过引入基因改造的供体菌,实现实时原位观察基因水平转移的过程,并可对转移频率和转移结果加以分析。通过对基因转移图像处理进行计算,得到微流控芯片通道内的基因转移率为(9.87±1.56)%,流式细胞分析结果表明转移接合子的细胞数量占芯片上混合菌的细胞数比例为0.21%(子占芯片上)。

To realize the real-time and in-situ observation of the gene transfer process, one experimental methodwas designed. Bythe inoculation of microfluidic chip donor and receptor bacteria as experimental carrier, and the introduction of donor gene transformation, real-time in situ observation process of horizontal gene transfer can be carried out, and the transfer frequency and transfer results also can be analyzed. The transfer rate in the channel of microfluidic chipcalculated from processed images is （9.87±1.56）%, and the proportion of transconj ugants got through flow cytometry analysis is （0. 21±0. 09）%.