黄柏及其不同炮制品的指纹图谱研究

Fingerprint Analysis Among Different Processed Products of Phellodendri Chinensis Cortex

目的:建立不同市售区黄柏生品及其蜜炙品、盐炙品、酒炙品的HPLC指纹图谱研究,比较不同市售区黄柏及其炮制品的指纹特征的差异。方法:采用HPLC法得出10批黄柏生品及其蜜炙品、盐炙品、酒炙品的指纹图谱并进行比较,流动相为乙腈(A)-水(含0.3%磷酸和0.3%二乙胺,B),梯度洗脱(0~10min,10%~14%A;10~20min,14%~25%A;20~45min,25%A),流速0.8mL/min,检测波长284nm,柱温40℃,分析时间45min。结果:蜜炙品、盐炙品、酒炙品与黄柏生品的指纹图谱基本一致,但各成分间的相对含量有所不同,对其中的绿原酸、黄柏碱、木兰花碱、盐酸药根碱、小檗红碱、盐酸巴马汀、盐酸小檗碱等成分进行了归属。10批黄柏生品及其蜜黄柏、盐黄柏、酒黄柏的共有模式色谱峰相似度都>0.9。结论:建立的指纹图谱有良好的精密度、稳定性和重复性,对黄柏生品及其不同炮制品的质量评价具有重要意义。

Objective：To develop an HPLC fingerprint analysis study of Phellodendri Chinensis Cortex and compare fingerprint differences of its processed products and the raw that purchasing from different places. Methods： HPLC was employed to compare fingerprint of ten batches of Phellodendri Chinensis Cortex and its different processed products with mobile phase consisting acetonitril （A） water（containing 0.3% phosphoric acid and 0.3% diethylamine, B） for gradient elution（0~ 10min, 10% ~ 14%A; 10~ 20min, 14 % ~25 %A; 20~ 45min, 25% A）, flow rate is 0.8mL/min, detection wavelength is set at 284 nm, column temperature is 40℃ and analytic time is 45 min. Results：Its processed products had a similer fingerprint chromatogram to the raw of Phellodendri Chinensis Cortex,but having a little diffrence in relative content of each component. Some peaks of the HPLC ehromatogram were identified as chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, berberrabine, palmatinen chloride and berberine hydrochloride etc. Similarities of total mode peaks among ten batches of raw materials, honey, salt and wine processed products were more than 0. 9. Conclusion. The established fingerprint has good precision, stability and reproducibility, which has an important sense of controlling the quality of Phellodendri Chinensis Cortex and its processed products.