整合REV LTR的自然重组马立克氏病毒GX0101的全基因组序列分析

Genomic Sequence Analysis of a Field Marek's Disease Virus Strain GX0101 with a Reticuloendotheliosis Virus Terminal Repeat Long Insert

GX0101是一株整合禽网状内皮组织增生症病毒(REV)长末端重复序列(LTR)的重组马立克氏病病毒(MDV)。基于MDV GX0101的细菌人工染色体感染性克隆利用高通量测序技术完成了对其全基因组的序列分析。GX0101全基因组长178101 bp,其基因组的TRL、UL、IRL、IRS、US和TRS区分别长12758 bp、113572 bp、12741 bp、12700 bp、11695 bp、13134 bp。与Md5不同,GX0101含有一个sorf2基因。同时,GX0101全基因组含有一个REV LTR重组片段,位于其基因组US区的sorf1中,sorf2基因前267 bp碱基处。通过与已发表的近10株MDV的比较,发现GX0101与英国分离株C12/130的两个不同毒力的感染性克隆p C12/130-10和p C12/130-15同源性最高。GX0101的全基因组序列的比较分析,有助于进一步阐明MDV致病性、传播性相关的基因,也有助于揭示不同地域间MDV的遗传变异和演化关系。

GX0101 is a recombinant Marek＇s disease virus （MDV） field strain with one reticuloendotheliosis virus （REV） long terminal repeat （LTR） insert. Based on the purified plasmid DNA of GX0101 bacterial artificial chromosome （BAC） infectious clone, the complete genome of GX0101 was sequenced and analyzed using high throughput sequencing technology. The complete genome sequence of GX0101 is 178101 bp in length. The terminal repeat long （TRL）, unique long （UL）, internal repeat long （IRL）, internal repeat short （IRS）, unique short （ US）, and terminal repeat short （TRS） regions were 12758 bp, 113572 bp, 12741 bp, 12700 bp, 11695 bp and 13134 bp, respectively. The GX0101 genome contains only one sorf2 gene, which is different from Md5. GX0101 contains a REV LTR insert located in sorfl gene of US region, at a site 267 bp upstream of the sorf2 gene in the genome. Among the 10 recently reported MDV strains, whole genome of GX0101 shows the highest identity to two BAC clones PC12130-10 and PC12130-15 of United Kingdom strain C12130 with different virulence. Analysis of the complete sequence of GX0101 will be useful on both studies of gene functions related to pathogenicity or transmission ability, and evolutionary relationships of MDVs in different geographical areas.