摘要
为研究猪霍乱沙门氏菌C79102株的菌种特性,制备了一批猪霍乱沙门氏菌C79102株,并对菌种的真空度、纯粹、剩余水分、培养特性、形态及生化特性、血清学特性、特异性、毒力等进行检定,重点对菌种的毒力进行研究。试验结果表明,该冻干菌种的真空度、纯粹、剩余水分、培养特性、形态及生化特性、血清学特性均符合《中华人民共和国兽用生物制品规程》二〇〇〇版质量标准的规定,特异性检验结果显示C79102在含1%醋酸铊的普通肉汤中不生长,毒力试验结果显示,不同制备方法获得的肉肝胃消化汤培养的菌液毒力差异明显。本研究可以为猪霍乱沙门氏菌的制备和检定提供参考依据。
In order to study the bacterial characteristics of Salmonella choleraesuis C79102 strain, a batch of Salmonella choleraesuis strain C79102 was prepared, and then the vacuum degree, residual moisture, morphology, culture characteristics, biochemical properties, serological characteristics, virulence, and immunogenicity were tested. The results showed that the vacuum degree, purity test result, residual moisture, morphology, culture characteristics, biochemical properties, serological characteristics of C79102 conformed to the Veterinary Biological Products Regulations of the People/s Republic of China, 2000 edition, Specificity test result showed that C79102 could not grow in common broth with 1% acetic acid thallium. The virulence test results showed that the virulence of C79102 obviously affected by meat liver stomach digested nmediumls quality. This research could provide some reference data to the preparation of Salmonella choleraesuis strain C79102
出处
《中国兽药杂志》
北大核心
2017年第10期24-28,共5页
Chinese Journal of Veterinary Drug
