辣蓼黄酮中芦丁、槲皮素与槲皮苷的含量分析

Determination of Contents of Rutin,Quercetin and Quercitrin in Flavonoids from Polygonum hydropiper

为保障辣蓼黄酮工业化生产中的质量控制,建立辣蓼黄酮中芦丁、槲皮素与槲皮苷含量测定高效液相色谱法。结果表明,运用高效液相色谱法测定辣蓼黄酮乙酸乙酯部分(FEA)中芦丁、槲皮苷与槲皮素的含量分别为281.97、160.98、83.31 mg/g,辣蓼黄酮正丁醇部分(FNB)中芦丁、槲皮苷与槲皮素的含量分别为150.33、147.51、73.82 mg/g。该方法简单、准确、灵敏、重复性好,可作为辣蓼黄酮中芦丁、槲皮素与槲皮苷含量分析的参考方法。

The flavonoids of Polygonum hydropiper were separated and purified by means of macroporous adsorption resin, and its antioxidant activity was tested in previous research, which gained satisfied results. In order to obtain flavonoids of Polygonum hydropipe with stable quality, and provide evidence for quality control in industrial production, high performance liquid chromatography for determination of total flavonoids in Polygonum hydropiper was used with rutin, quercetin and quercitrin as reference substance. The results showed that the contents of rutin, quercetin and quercitrin from flavonoids ethyl acetate （FEA） in Polygonum hydropipe were 281. 97, 160.98, 83.31 mg/g respectively; The contents of rutin, quercetin and quercitrin of flavonoids n-butanol （FNB） in polygonum hydropiper were 150.33, 147.51, 73.82 mg/g respectively. The method demonstrated advantage of simply, accuraty and sensitivity, and successfully applied to the analysis of rutin, quercetin and quercitrin content in flavonoids from Polygonum hydropipe.