土壤做培养基支撑物对无核枣试管苗生根培养的影响

Effect of Soil Supporting Medium on Rooting of Stoneless Chinese Jujube Plantlet in Vitro

在土壤做支撑物的培养基中接种无核枣试管苗茎段进行培养,探讨土壤支撑培养基对无核枣试管苗生根的影响。结果表明:培养基中含有不添加大量元素的MS培养基成分、15 g/L蔗糖和0.4 mg/L IBA时,可以形成正常的试管苗,虽然试管苗茎长和根长等指标明显低于琼脂支撑培养对照,但试管苗生根率明显高于对照,并达到95.8%。在土壤支撑的培养基中添加大量元素对无核枣试管苗的生根与生长有明显抑制作用,添加蔗糖则有明显的促进作用。该研究结果可降低无核枣试管苗生产成本,并为其移栽的简化奠定基础。

The stem sections of stoneless Chinese Jujube plantlet in vitro were inoculated and cultured in soil supporting medium to research the effect of soil supporting medium on rooting of stoneless Chinese jujube micro-shoot. The result show that in the medium containing MS medium composition without macro-element, 15 g/L of cane sugar and 0.4 mg/L of IBA the stem section formed normal plantlet whose stem length and root length were significantly lower than that of the plantlet formed in agar supporting medium but rooting rate was 95.8% which was obvious higher than that of the plantlet formed in agar supporting medium. Adding macro-element in soil supporting medium had inhibitory action but adding cane sugar had promoting action for rooting and growth of stonetess Chinese Jujube plantlet in vitro. These results can reduce the cost of stoneless Chinese Jujube plantlet in vitro and provide a basis for simplify the transplanting procedure of the plantlet.