天津地区副溶血弧菌分离株血清型、部分毒力基因的分布和耐药性调查

Identification of Serotype, Main Virulence Factors and Investigation of Drug-resistant of Vibrio Parahaemolyticus Strains from Tianjin

为了解天津市水产品中副溶血弧菌的主要血清型、毒力因子携带情况和耐药性情况。调查了2015年天津地区海产品中副溶血弧菌的污染状况,并分析了不同来源的副溶血弧菌主要毒力基因TDH、TRH的分布情况及血清分型,对不同血清型的副溶血弧菌进行了耐药性试验。2015年从天津地区9个区县采集样品共计73份,共分离出38株副溶血弧菌,分离率达52%,毒力相关基因分析结果显示,TDH基因携带率为13.2%,TRH阳性率为5.3%;血清分型结果显示,共分出O1、O3、O10三个血清型,其中O1血清型占60%,为主要的血清型;对不同血清型进行了9种药物的MIC试验,结果显示,对喹诺酮类(烟酸诺氟沙星、乳酸诺氟沙星)比较敏感,对磺胺类的磺胺二甲嘧啶、磺胺嘧啶钠、新诺明和氨基糖苷类的硫酸新霉素不敏感,对其他抗生素也产生不同程度的耐药,不同血清型的副溶血弧菌对药物的敏感性没有显著差异,在治疗该菌引起的疾病时可以不考虑血清型。结果提示,天津地区海产品副溶血弧菌检出率较高,部分菌株携带毒力基因,研究结果可为深入研究副溶血弧菌的致病性提供基础数据。

To investigate the serotype, toxic genes and antibiotics resistance of Fibrio Parahaemolyticus from the seafoods in Tianjin. Several aquatic species and enviroments were examined for presence of V. Parahaemolyticus in Tianjin of 2015, and some virulence-related genes such as tdh, trh and serotype were investigated from the isolates, the drug-resistant test was carried out to the different serum type strains of V.P. In 2015, 73 samples from 9 districts and countries were collected from Tianjin, and a total of 38 strains of V. Parahaemolyticus were isolated. The isolation rate about 52% the genes tdh, trh existed in 13.2% and 5.2% respectively from 38 isolates; The results of serum typing showed that O1, 03 and O10 were separated, and including serotype O1 accounting for 60% of the major serotype. MIC test was carried out on 9 kinds of drugs with different serum type, the result showed that II. parahaemolyticus was sensitived to quinolones （nicotinic acid norfloxacin Injection, norfloxacin lactate）, and non-sensitived to sulfadimidine, sulfadiazine sodium, sulfamethoxazole and neomycin sulphate, and other antibiotics also have different degrees of resistance, There was no significant difference between the sensitivity of I1. parahaemolyticus in different serotypes of drugs, in the treatment of diseases caused by the strains could not be considered serotype. This study reveals the significant prevalence of 1I. parahaemolyticus in seafoods with high diversity of virulence genes. Representative V. parahaemolyticus isolates could be used to provide basic data for the further study of the pathogenicity of IT. parahaemolyticus.