Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理研究

Clinicopathological study of Fas-related death domain protein expression in breast cancer

目的探讨Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理特点。方法选择2013年2月~2017年2月我院收治的行乳腺肿瘤手术治疗患者的人体石蜡包埋组织标本148例,其中乳腺良性病变26例(A组),无淋巴结转移乳腺癌58例(B组),淋巴结转移乳腺癌64例(C组)。所有标本均实施免疫组化检查,分析Fas相关死亡结构域蛋白表达。结果 Fas相关死亡结构域蛋白表达于乳腺癌癌细胞胞浆内和乳腺良性病变上皮细胞内。B组中Fas相关死亡结构域蛋白表达较弱,染色范围较小,染色深度均较低;A组、C组中Fas相关死亡结构域蛋白表达较弱,染色深度强。A、B、C三组Fas相关死亡结构域蛋白阳性表达率分别为88.5%(23/26)、46.6%(27/58)、65.6%(42/64),A组高于B组、C组,差异有统计学意义(P<0.05)。在乳腺癌患者中,淋巴结转移、确诊年龄与Fas相关死亡结构域蛋白表达有关(P<0.05),但临床分期、组织学类型与Fas相关死亡结构域蛋白表达无关(P>0.05)。结论 Fas相关死亡结构域蛋白与乳腺癌淋巴结转移、确诊年龄有着密切的关系,但与临床分期、组织学类型无关,需引起高度关注。

Objective To explore the clinicopathological features of Fas-related death domain proteins expression in breast cancer.Methods From February 2013 to February 2017,148 cases of paraffin-embedded tissue specimens from patients undergone tumor surgeries were selected,There were benign breast lesions in 26 cases （group A）,breast cancer without lymph node metastasis in 58 cases （group B）,and breast cancer with lymph node metastasis in 64 cases （group C）.All specimens were performed with immunohistochemical examinations in order to analyze the proteins expression of Fas-related death domain.Results It was detected that Fas-related death domain proteins were expressed in the intra- cytoplasm of breast cancer cells and epithelial cells of benign breast lesions.In group B,the expression of Fas-related death domain proteins was weak and dyeing range and depth were small compared with those in groups of A and C, which were in slight expression mad deep dyeing.The positivity expression rates of Fas-related death domain proteins in groups of A,B,and C were 88.5% （23/26）,46.6% （27/58）,and 65.6% （42/64） respectively.The positivity expression rate in group A was higher than that in groups of B and C with statistical differences （P〈0.O5）.For breast cancer patients,lymph node metastasis and age with a definite diagnosis were associated with Fas-related death domain protein expression （P〈 0.05）.However,the clinical stage and histological type were not related to the expression of Fas-related death domain protein （P〉0.05）.Conclusion The expression of Fas-related death domain proteins is closely related to the lymph node metastasis and age with a definite diagnosis of breast cancer,but it has nothing to do with the clinical stage and histo- logical type,to which should be paid high attention.