体外评估多烯紫杉醇与顺铂给药顺序依赖性的相互作用(英文)

In Vitro Evaluation of Schedule-dependent Interaction between Docetaxel and Cisplatin against Human Breast Cancer Cells

目的 :将多烯紫杉醇与顺铂以不同给药顺序处理人乳腺癌细胞研究两者细胞毒性的相互作用。方法 :应用一系列体外实验如DNA片段分析、MTT测定、流式细胞仪分析和蛋白印迹法分析两者以不同给药顺序的实验结果。结果 :显示当同时给药或顺铂在多烯紫杉醇前给药则发生拮抗作用 ;多烯紫杉醇在顺铂前给药则不发生拮抗作用。结论 :顺铂干扰多烯紫杉醇对癌细胞的捕获和细胞凋亡 ,并影响多烯紫杉醇诱导的bcl 2磷酸化 ;

OBJECTIVE:To study the schedule dependent interactions between docetaxel and cisplatin against human breast cancer cells.METHOD:The assays in vitro were used including DNA fragmentation analyse, MTT assay, flow cytometric analyses, and western blotting.RESULTS:The antagonistic interaction occurred when tumor cells were exposed to the two drugs simultaneously or exposed to cisplatin before docetaxel. No antagonism was observed when docetaxel was added before cisplatin.CONCLUSIONS:Cisplatin could interfere with the cytotoxic effects of docetaxel on both mitotic arrest and apoptosis. In addition, the combination of two drugs could inhibit the bcl 2 phosphorylation that induced by docetaxel. These results indicate that interaction between docetaxel and cisplatin is highly schedule dependent.